Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-28067 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- COX1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: 293T, A431, HepG2, Molt-4, Raji.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using COX1 Polyclonal Antibody (Product # PA5-28067). Sample (30 µg of whole cell lysate). A: MOLT4SDS PAGE. Z. 10% SDS PAGE. COX1 Polyclonal Antibody (Product # PA5-28067) diluted at 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-COX1/PTGS1 Polyclonal Antibody (Product # PA5-28067) and bands at 71, 56 kDa corresponding to COX1/PTGS1 were observed across the cell lines and tissues tested along with an uncharacterized band (*) at ~35 kDa. Whole cell extracts (30 µg lysate) of MCF10A (Lane 1), MCF7 (Lane 2), K562 (Lane 3), CACO2 (Lane 4), HT-29 (Lane 5), tissue extracts (30 µg lysate) of Mouse Brain (Lane 6) and Mouse Liver (Lane 7) were electrophoresed using NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded PC13 xenograft, using PTGS1 (Product # PA5-28067) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.