Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunoprecipitation [3]
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Validation data
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- Product number
- LS-C502063 - Provider product page
- Provider
- LSBio
- Product name
- USE1 / p31 Antibody LS-C502063
- Antibody type
- Polyclonal
- Description
- Protein G purified
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Short term: -20°C; Long term: -80°C; Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Positive WB detected in:Jurkat whole cell lysate,Mouse heart tissue,Mouse brain tissue,Mouse kidney tissue,Mouse stomach tissue,Mouse skeletal muscle tissue;All lanes:USE1 antibody at 2.8ug/ml;Secondary;Goat polyclonal to rabbit IgG at 1/50000 dilution;Predicted band size: 30,17,16 kDa;Observed band size: 36 kDa;
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunoprecipitating USE1 in PC-3 whole cell lysate Lane 1: Rabbit control IgG (1µg) instead of USE1 Antibody in PC-3 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: USE1 Antibody (6µg) + PC-3 whole cell lysate (500µg) Lane 3: PC-3 whole cell lysate (10µg)
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunoprecipitating USE1 in PC-3 whole cell lysate Lane 1: Rabbit control IgG (1µg) instead of USE1 Antibody in PC-3 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: USE1 Antibody (6µg) + PC-3 whole cell lysate (500µg) Lane 3: PC-3 whole cell lysate (10µg)
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunoprecipitating USE1 in PC-3 whole cell lysate Lane 1: Rabbit control IgG (1µg) instead of USE1 Antibody in PC-3 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: USE1 Antibody (6µg) + PC-3 whole cell lysate (500µg) Lane 3: PC-3 whole cell lysate (10µg)