PA1-37504

antibody from Invitrogen Antibodies

Targeting: PTGS2 COX2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

PA1-37504

Provider

Invitrogen Antibodies

Product name

Anti-COX2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody is predicted to react with bovine, mouse and rabbit based on sequence homology. Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is colon, lung or breast carcinoma.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

7 mL

Storage

4° C, do not freeze

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-COX2 Rabbit Polyclonal Antibody (Product # PA1-37504) and a 74 kDa band corresponding to COX2 was induced in RAW 264.7 upon LPS treatment. Membrane enriched extracts (30 µg lysate) of RAW 264.7 (Lane 1) and RAW 264.7 treated with LPS (1µg/ml for 24hr) (Lane 2) were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:3000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005)..