Antibody data
- Antibody Data
- Antigen structure
- References [12]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- PB9148 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-MyD88 PTGS2 Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Polyclonal antibody for MYD88 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB, IHC-P, IHC-F, ICC, IF, FCM, ELISA(Cap). Reactive species: Human. MYD88 information: Molecular Weight: 68996 MW; Subcellular Localization: Microsome membrane; Peripheral membrane protein. Endoplasmic reticulum membrane; Peripheral membrane protein; Tissue Specificity: Ubiquitous.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references Chaihu Guizhi Ganjiang Decoction attenuates nonalcoholic steatohepatitis by enhancing intestinal barrier integrity and ameliorating PPARα mediated lipotoxicity.
Mechanism of Salvia miltiorrhiza Bunge extract to alleviate Chronic Sleep Deprivation-Induced cognitive dysfunction in rats.
Three Artemisia pollens trigger the onset of allergic rhinitis via TLR4/MyD88 signaling pathway.
Active fractions from Jingfang Baidu Powder alleviate Klebsiella-induced Pneumonia by inhibiting TLR4/Myd88-ERK signaling pathway.
Lutein Prevents Liver Injury and Intestinal Barrier Dysfunction in Rats Subjected to Chronic Alcohol Intake.
UHPLC-MS-Based Metabolomics Reveal the Potential Mechanism of Armillaria mellea Acid Polysaccharide in and Its Effects on Cyclophosphamide-Induced Immunosuppressed Mice.
Leonurine Preconditioning Attenuates Ischemic Acute Kidney Injury in Rats by Promoting Nrf2 Nuclear Translocation and Suppressing TLR4/NF-κB Pathway.
eIF3k inhibits NF-κB signaling by targeting MyD88 for ATG5-mediated autophagic degradation in teleost fish.
Anti-Inflammatory Activity of Mulberry Leaf Flavonoids In Vitro and In Vivo.
Gastroprotective Effects of Periplaneta americana L. Extract Against Ethanol-Induced Gastric Ulcer in Mice by Suppressing Apoptosis-Related Pathways.
Suppression of the toll-like receptor 7-dependent type I interferon production pathway by autophagy resulting from enterovirus 71 and coxsackievirus A16 infections facilitates their replication.
Puerarin protects brain tissue against cerebral ischemia/reperfusion injury by inhibiting the inflammatory response.
Wu H, Lou T, Pan M, Wei Z, Yang X, Liu L, Feng M, Shi L, Qu B, Cong S, Chen K, Yang H, Liu J, Li Y, Jia Z, Xiao H
Journal of ethnopharmacology 2024 May 23;326:117841
Journal of ethnopharmacology 2024 May 23;326:117841
Mechanism of Salvia miltiorrhiza Bunge extract to alleviate Chronic Sleep Deprivation-Induced cognitive dysfunction in rats.
Yin C, Zhang M, Jin S, Zhou Y, Ding L, Lv Q, Huang Z, Zhou J, Chen J, Wang P, Zhang S, You Q
Phytomedicine : international journal of phytotherapy and phytopharmacology 2024 Jul 25;130:155725
Phytomedicine : international journal of phytotherapy and phytopharmacology 2024 Jul 25;130:155725
Three Artemisia pollens trigger the onset of allergic rhinitis via TLR4/MyD88 signaling pathway.
Zhang J, Gao L, Yu D, Song Y, Zhao Y, Feng Y
Molecular biology reports 2024 Feb 23;51(1):319
Molecular biology reports 2024 Feb 23;51(1):319
Active fractions from Jingfang Baidu Powder alleviate Klebsiella-induced Pneumonia by inhibiting TLR4/Myd88-ERK signaling pathway.
Sun C, Xu Y, Xu G, Ji X, Jiang P, He Y
Journal of ethnopharmacology 2024 Aug 10;330:118067
Journal of ethnopharmacology 2024 Aug 10;330:118067
Lutein Prevents Liver Injury and Intestinal Barrier Dysfunction in Rats Subjected to Chronic Alcohol Intake.
Zhao S, Zhang Y, Ding H, Hu S, Wu X, Ma A, Ma Y
Nutrients 2023 Feb 28;15(5)
Nutrients 2023 Feb 28;15(5)
UHPLC-MS-Based Metabolomics Reveal the Potential Mechanism of Armillaria mellea Acid Polysaccharide in and Its Effects on Cyclophosphamide-Induced Immunosuppressed Mice.
Li Y, Li Q, Niu H, Li H, Jiao L, Wu W
Molecules (Basel, Switzerland) 2023 Dec 5;28(24)
Molecules (Basel, Switzerland) 2023 Dec 5;28(24)
Leonurine Preconditioning Attenuates Ischemic Acute Kidney Injury in Rats by Promoting Nrf2 Nuclear Translocation and Suppressing TLR4/NF-κB Pathway.
Han L, Chen A, Liu L, Wang F
Chemical & pharmaceutical bulletin 2022;70(1):66-73
Chemical & pharmaceutical bulletin 2022;70(1):66-73
eIF3k inhibits NF-κB signaling by targeting MyD88 for ATG5-mediated autophagic degradation in teleost fish.
Chen Y, Cao B, Zheng W, Sun Y, Xu T
The Journal of biological chemistry 2022 Mar;298(3):101730
The Journal of biological chemistry 2022 Mar;298(3):101730
Anti-Inflammatory Activity of Mulberry Leaf Flavonoids In Vitro and In Vivo.
Lin Z, Gan T, Huang Y, Bao L, Liu S, Cui X, Wang H, Jiao F, Zhang M, Su C, Qian Y
International journal of molecular sciences 2022 Jul 12;23(14)
International journal of molecular sciences 2022 Jul 12;23(14)
Gastroprotective Effects of Periplaneta americana L. Extract Against Ethanol-Induced Gastric Ulcer in Mice by Suppressing Apoptosis-Related Pathways.
Fu S, Chen J, Zhang C, Shi J, Nie X, Hu Y, Fu C, Li X, Zhang J
Frontiers in pharmacology 2021;12:798421
Frontiers in pharmacology 2021;12:798421
Suppression of the toll-like receptor 7-dependent type I interferon production pathway by autophagy resulting from enterovirus 71 and coxsackievirus A16 infections facilitates their replication.
Song J, Hu Y, Li J, Zheng H, Wang J, Guo L, Shi H, Liu L
Archives of virology 2018 Jan;163(1):135-144
Archives of virology 2018 Jan;163(1):135-144
Puerarin protects brain tissue against cerebral ischemia/reperfusion injury by inhibiting the inflammatory response.
Zhou F, Wang L, Liu P, Hu W, Zhu X, Shen H, Yao Y
Neural regeneration research 2014 Dec 1;9(23):2074-80
Neural regeneration research 2014 Dec 1;9(23):2074-80
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- Western blot analysis of MYD88 using anti-MYD88 antibody (PB9148). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Recombinant Human MYD88 Protein 0.5ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MYD88 antigen affinity purified polyclonal antibody (Catalog # PB9148) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MYD88 at approximately 49KD. The expected band size for MYD88 is at 49KD.
- Additional image