M00084

antibody from Boster Biological Technology

Targeting: PTGS2 COX2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Antibody Data

Product number

M00084

Provider

Boster Biological Technology

Product name

Anti-COX2 PTGS2 Rabbit Monoclonal Antibody

Antibody type

Monoclonal

Description

Monoclonal antibody for COX 2/PTGS2 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: IF, ICC, WB. Reactive species: Human, Mouse COX 2/PTGS2 information: Molecular Weight: 68996 MW; Subcellular Localization: Microsome membrane; Peripheral membrane protein. Endoplasmic reticulum membrane; Peripheral membrane protein.

Reactivity

Human, Mouse

Host

Rabbit

Antibody clone number

EEC-16

Vial size

100ug/vial

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

At -20°C for one year. Avoid repeated freezing and thawing.

References

Submitted references

A breakthrough in periodontitis treatment: Revealing the pharmacodynamic substances and mechanisms of Kouqiangjie formula.

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Journal of ethnopharmacology 2024 Apr 6;323:117738

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Macromolecular nanoparticles to attenuate both reactive oxygen species and inflammatory damage for treating Alzheimer's disease.

Zhang B, Zhao Y, Guo K, Tian H, Wang C, Wang R, Chen Y, Chen X, Zheng H, Gao B, Shen J, Tian W
Bioengineering & translational medicine 2023 May;8(3):e10459

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Combinatorial treatment with Silybum marianum essential oil enhances the therapeutic efficacy of a 5-fluorouracil base therapy for hepatocellular carcinoma.

MasodKhooy MJ, Farasat M, Salehi Salmi M, Mirzaei H
Phytotherapy research : PTR 2023 May;37(5):1968-1985

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miR-210/NF-κB axis: A new direction for regulating cadmium-induced pig artery inflammatory injury.

Zhang Y, Liu Q, Wu H, Du Y, Wang X, Xu S
Journal of cellular physiology 2023 Jul;238(7):1605-1621

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Secoisolariciresinol diglucoside Ameliorates Osteoarthritis via Nuclear factor-erythroid 2-related factor-2/ nuclear factor kappa B Pathway: In vitro and in vivo experiments.

Zhang Z, Wang S, Liu X, Yang Y, Zhang Y, Li B, Guo F, Liang J, Hong X, Guo R, Zhang B
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Ketorolac tromethamine represses senescence in aging articular chondrocytes.

Zhang W, Mei R, Wu S
Bioscience, biotechnology, and biochemistry 2022 Jun 25;86(7):837-845

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An Acidic Polysaccharide with Anti-Inflammatory Effects from Blackened Jujube: Conformation and Rheological Properties.

Liu C, Wang F, Zhang R
Foods (Basel, Switzerland) 2022 Aug 17;11(16)

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Ding's herbal enema treats dextran sulfate sodium-induced colitis in mice by regulating the gut microbiota and maintaining the Treg/Th17 cell balance.

Tan YY, Ding Y, Zheng X, Dai GJ, Zhang SM, Yang X, Xu DC, Chen P, Zhang JM, Ma JZ, Li M, Huang SC, Liu Y, Zhang YT, Xing H, Ding K, Ding YJ
Experimental and therapeutic medicine 2021 Dec;22(6):1368

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Nifedipine inhibits oxidative stress and ameliorates osteoarthritis by activating the nuclear factor erythroid-2-related factor 2 pathway.

Yao J, Long H, Zhao J, Zhong G, Li J
Life sciences 2020 Jul 15;253:117292

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Nephroprotective Effects of Saponins from Leaves of Panax quinquefolius against Cisplatin-Induced Acute Kidney Injury.

Ma ZN, Li YZ, Li W, Yan XT, Yang G, Zhang J, Zhao LC, Yang LM
International journal of molecular sciences 2017 Jul 13;18(7)

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Anti-inflammatory and anti-nociceptive activities of methanol extract from aerial part of Phlomis younghusbandii Mukerjee.

Wang QS, Yang L, Cui WY, Chen L, Jiang YH
PloS one 2014;9(3):e89149

Western blot

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Western blot analysis of COX2 expression in RAW2647 cell lysate treated with LPS (M00084). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-COX2 monoclonal antibody (Catalog # M00084) overnight at 4

Additional image