Antibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- A00084-2 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-COX2/Cyclooxygenase 2/PTGS2 Picoband™ Antibody
- Antibody type
- Polyclonal
- Description
- Rabbit IgG polyclonal antibody for COX2/Cyclooxygenase 2/PTGS2 detection. Tested with WB, FCM, Direct ELISA in Human.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references Combinatorial treatment with Silybum marianum essential oil enhances the therapeutic efficacy of a 5-fluorouracil base therapy for hepatocellular carcinoma.
Secoisolariciresinol diglucoside Ameliorates Osteoarthritis via Nuclear factor-erythroid 2-related factor-2/ nuclear factor kappa B Pathway: In vitro and in vivo experiments.
Old targets, new strategy: Apigenin-7-O-β-d-(-6″-p-coumaroyl)-glucopyranoside prevents endothelial ferroptosis and alleviates intestinal ischemia-reperfusion injury through HO-1 and MAO-B inhibition.
An Acidic Polysaccharide with Anti-Inflammatory Effects from Blackened Jujube: Conformation and Rheological Properties.
The protective effects of maltol on cisplatin-induced nephrotoxicity through the AMPK-mediated PI3K/Akt and p53 signaling pathways.
Anti-inflammatory and anti-nociceptive activities of methanol extract from aerial part of Phlomis younghusbandii Mukerjee.
MasodKhooy MJ, Farasat M, Salehi Salmi M, Mirzaei H
Phytotherapy research : PTR 2023 May;37(5):1968-1985
Phytotherapy research : PTR 2023 May;37(5):1968-1985
Secoisolariciresinol diglucoside Ameliorates Osteoarthritis via Nuclear factor-erythroid 2-related factor-2/ nuclear factor kappa B Pathway: In vitro and in vivo experiments.
Zhang Z, Wang S, Liu X, Yang Y, Zhang Y, Li B, Guo F, Liang J, Hong X, Guo R, Zhang B
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2023 Aug;164:114964
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2023 Aug;164:114964
Old targets, new strategy: Apigenin-7-O-β-d-(-6″-p-coumaroyl)-glucopyranoside prevents endothelial ferroptosis and alleviates intestinal ischemia-reperfusion injury through HO-1 and MAO-B inhibition.
Feng YD, Ye W, Tian W, Meng JR, Zhang M, Sun Y, Zhang HN, Wang SJ, Wu KH, Liu CX, Liu SY, Cao W, Li XQ
Free radical biology & medicine 2022 May 1;184:74-88
Free radical biology & medicine 2022 May 1;184:74-88
An Acidic Polysaccharide with Anti-Inflammatory Effects from Blackened Jujube: Conformation and Rheological Properties.
Liu C, Wang F, Zhang R
Foods (Basel, Switzerland) 2022 Aug 17;11(16)
Foods (Basel, Switzerland) 2022 Aug 17;11(16)
The protective effects of maltol on cisplatin-induced nephrotoxicity through the AMPK-mediated PI3K/Akt and p53 signaling pathways.
Mi XJ, Hou JG, Wang Z, Han Y, Ren S, Hu JN, Chen C, Li W
Scientific reports 2018 Oct 29;8(1):15922
Scientific reports 2018 Oct 29;8(1):15922
Anti-inflammatory and anti-nociceptive activities of methanol extract from aerial part of Phlomis younghusbandii Mukerjee.
Wang QS, Yang L, Cui WY, Chen L, Jiang YH
PloS one 2014;9(3):e89149
PloS one 2014;9(3):e89149
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- Western blot analysis of PTGS2 using anti-PTGS2 antibody (A00084-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 22RV1 whole cell lysates, Lane 3: human U-87MG whole cell lysates, Lane 4: human A549 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTGS2 antigen affinity purified polyclonal antibody (Catalog # A00084-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PTGS2 at approximately 75KD. The expected band size for PTGS2 is at 69KD.
- Additional image