- PA1-9032 - Invitrogen Antibodies PTGS2 antibody

Submitted references Sulindac acetohydrazide derivative attenuates against cisplatin induced organ damage by modulation of antioxidant and inflammatory signaling pathways.

Razak S, Afsar T, Bibi N, Abulmeaty M, Bhat MA, Inam A, Trembley JH, Almajwal A, Shabbir M, Alruwaili NW, Algarni A
Scientific reports 2022 Jul 11;12(1):11749

Molecular docking, pharmacokinetic studies, and in vivo pharmacological study of indole derivative 2-(5-methoxy-2-methyl-1H-indole-3-yl)-N'-[(E)-(3-nitrophenyl) methylidene] acetohydrazide as a promising chemoprotective agent against cisplatin induced organ damage.

Razak S, Afsar T, Bibi N, Abulmeaty M, Qamar W, Almajwal A, Inam A, Al Disi D, Shabbir M, Bhat MA
Scientific reports 2021 Mar 18;11(1):6245

Korean Red Ginseng affects ovalbumin-induced asthma by modulating IL-12, IL-4, and IL-6 levels and the NF-κB/COX-2 and PGE(2) pathways.

Lee SY, Kim MH, Kim SH, Ahn T, Kim SW, Kwak YS, Cho IH, Nah SY, Cho SS, Park KM, Park DH, Bae CS
Journal of ginseng research 2021 Jul;45(4):482-489

Saururus chinensis-controlled allergic pulmonary disease through NF-κB/COX-2 and PGE(2) pathways.

Song M, Lee SY, Kim M, Park S, Park J, Kwon Y, Park DH
PeerJ 2020;8:e10043

Anti-inflammatory Effect of Curcuma longa and Allium hookeri Co-treatment via NF-κB and COX-2 Pathways.

Lee SY, Cho SS, Li Y, Bae CS, Park KM, Park DH
Scientific reports 2020 Mar 31;10(1):5718

No comments: Submit comment

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of COX2 by a COX2 monoclonal antibody (Product # PA1-9032) at a concentration of 0.1 µg/mL. A549 (A) and Daudi (B) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: CRISPR-Cas9 mediated genome editing ofCOX2 (as confirmed by next-generation sequencing) was achieved by electroporation with TrueGuide synthetic gRNA (Product # A35533, AssayID CRISPR568234_SGM) and LentiArray Cas9 Lentivirus (Product # A32064). Fig (a) Western blot analysis of COX2 was performed by loading 30 µg of RAW 264.7 wild type (Lane 1), RAW 264.7 wild type treated with 1 µg/mL LPS for 24 hours (Lane 2), RAW 264.7Cas9 cells electroporated with COX2 TrueGuide Synthetic gRNA (Lane 3) and RAW 264.7 Cas9 cells electroporated with COX2 TrueGuide Synthetic gRNAtype treated with 1 µg/mL LPS for 24 hours (Lane 4) membrane enriched extracts. The samples were electrophoresed using NuPAGE™ Novex™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with COX2 Polyclonal Antibody (Product # PA1-9032, 1:1000 dilution) and Rabbit anti-Goat IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27014, 1:5000 dilution).Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005). A loss of signal in sgRNA transduced cells using the LentiArray™ CRISPR product line confirms that antibody is specific toCOX2.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-COX2 Goat Polyclonal Antibody (Product # PA1-9032) and a 74 kDa band corresponding to COX2 was induced in RAW 264.7 upon LPS treatment. Membrane enriched extracts (30 µg lysate) of RAW 264.7 (Lane 1) and RAW 264.7 treated with LPS (1 µg/mL for 24hr) (Lane 2) were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.3 µg/mL) and detected by chemiluminescence with Rabbit anti-Goat IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27014, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of COX2 in HepG2 cells using a COX2 monoclonal antibody (Product # PA1-9032) at 10 µg/mL for1hr. The cells were paraformaldehyde fixed and permeabilized with 0.15% Triton. Primary incubation was followed by Alexa Fluor 488 secondary antibody (2 µg/mL) showing cytoplasmic/vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL)followed by Alexa Fluor 488 secondary antibody (2 µg/mL).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of COX2 in NIH3T3 cells using a COX2 monoclonal antibody (Product # PA1-9032) at 10 µg/mL for1hr. The cells were paraformaldehyde fixed and permeabilized with 0.15% Triton. Primary incubation was followed by Alexa Fluor 488 secondary antibody (2 µg/mL) showing cytoplasm and vesicle staining. The nuclear stain is DAPI (blue).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of COX2 was performed using 70% confluent log phase RAW 264.7 cells treated with 100 ng/mL of Lipopolysaccharide for 24 Hrs. The cells were fixed with 4% Paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 10 minutes at room temperature. The cells were labeled with COX2 Polyclonal Antibody (Product # PA1-9032) at 5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Rabbit anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 (Product # A-11078), (1:2000 dilution) for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing Cytoplasmic and nuclear membrane localization. Panel e represents untreated cells with reduced signal. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometric analysis of COX2 in HeLa cells using a COX2 monoclonal antibody (Product # PA1-9032) at 10 µg/mL for 1hr, depicted by the blue line. The cells were paraformaldehyde fixed and permeabilized with 0.5% Triton. Primary incubation followed by Alexa Fluor 488 secondary antibody (2 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 6 Saururus chinensis (SC) water-extract controlled inflammation through the NF-kappa B/COX-2 and PGE 2 axis. (A-P) Saururus chinensis (SC) water-extract inhibited COX-2 expression via preventing NF-kappa B translocation. Compared to the results in ovalbumin and fine dust treatment group in tap water treatment group the expression of COX-2 protein (red spot) was decreased significantly. In ovalbumin and fine dust treatment group NF-kappa B proteins were translocated from cytoplasm to nucleus and the expression of COX-2 protein was increased effectively. Both in dexamethasone treatment group and in 300 mg/kg SC treatment group, the proteins expression were down-regulated, both of NF-kappa B and COX-2. (R-I) SC effectively inhibited the PGE 2 expression. R, tap water treatment (control) group; S, ovalbumin and fine dust treatment (induction) group; T, ovalbumin, fine dust, and dexamethasone treatment (positive control) group; I, ovalbumin, fine dust, and 300 mg/kg SC treatment group. Arrow, NF-kappa B; arrowhead, COX-2. Magnification, 1000 x. Scale bar, 50 mu m. (Q) The quantitative score of NF- kappa B p65 vs. nucleus or COX-2 vs. nucleus. All the values are presented as mean +- standard deviation. n = 8 per each group. * p < 0.05 vs. tap water treatment group; $ p < 0.05 vs. ovalbumin and fine dust treatment group; $$ p < 0.001 vs. ovalbumin and fine dust treatment group. (V) The number of PGE 2 positive cells in the lung tissue. All values are presented as mean +- stan

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 2 Effect of MMINA on CD4 + COX2, CD4 + STAT3, and CD4 + TNF-alpha population. Flow cytometric analysis of CD4 + COX2, STAT3, TNF-alpha and CD4 + expressing cells in whole bllod from one rat from each group. Statistical analysis was performed using a one way ANOVA followed by the Tukey-Kramer post-test. Each value indicates the mean +- SEM of six animals.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 5 Western blot analysis of STAT3, COX2, NFkB p65, IL1 and TNF-alpha protein expression in ( a ) Kidney, ( b ) Liver, ( c ) Brain and ( d ) Heart tissue lysates from different treatment groups. Treatments are indicated above the blots. Full sized blots are included in the Supplementary file S3 . All gels and blots were run under the same experimental conditions as described in "" Methods "".

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 4 Effect of Korean Red Ginseng water extract (KRGWE) on translocation of NF-kappaBp65 from the cytoplasm to the nucleus . (A) KRGWE significantly inhibited the level of COX-2 expression in the cytoplasm through NF-kappaB translocation from the cytoplasm to the nucleus where it would act as a transcription factor of COX-2. Magnification 1000-fold. Scale bar indicates 50 mum. (B) According to the respective fluorescence, KRGWE downregulated NF-kappaB translocation and COX-2 expression. (C) KRGWE significantly suppressed PGE 2 expression, which was elicited by ovalbumin treatments. Magnification 200-fold. Scale bar indicates 100 mum; a: control group; b: ovalbumin-treated group; c: 1 mg/kg/day DEX treated group after ovalbumin treatment; d: 50 mg/kg/day KRGWE-treated group after ovalbumin treatment. Statistical significance is indicated as follows: * p < 0.05 vs. control group; ** p < 0.01 vs. control group; $ p < 0.05 vs. ovalbumin-treated group; $$ p < 0.01 vs. ovalbumin-treated group Fig. 4

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 4 C. longa and A. hookeri co-treatment inhibits NF-kappaB and COX-2 expression. ( A ) NF-kappaB is downregulated in the nucleus and COX-2 in the cytoplasm. NF-kappaB and COX-2 are not expressed in the control group and only the nucleus is stained with DAPI (blue spots). Carrageenan stimulates the expression of NF-kappaB in the nucleus and that of COX-2 in the cytoplasm. MSM suppresses the expression of NF-kappaB and COX-2 induced by carrageenan, but the expression of NF-kappaB (arrow) and COX-2 (arrowhead) observed is minimal. C. longa and A. hookeri co-treatment significantly inhibits the expression of NF-kappaB and COX-2. ( B ) The graph and scores present the immunofluorescent results using the image analyzing program in the K1-Fluo confocal microscope. MSM and C. longa and A. hookeri co-treatment statistically significantly control NF-kappaB and COX-2 expression effectively. ( C ) Compared to the density of NF-kappaB in the nucleus and cytoplasm, the pattern is similar to that observed in the immunofluorescence assay. C. longa and A. hookeri co-treatment blocks the translocation of NF-kappaB into the nucleus from the cytoplasm and inhibits the expression of COX-2 in the cytoplasm. ( D ) Depending on the image analysis of the western blotting of NF-kappaB (in nucleus and in cytoplasm) and COX-2 (in the skin tissue), the density graph based on each band per beta-actin was presented using the Image J program. ( E ) MSM and C. longa and A. hookeri co

PA1-9032

Antibody data