Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- R1185 - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#R1185, RRID:AB_979283
- Product name
- anti Cyclooxygenase 2
- Antibody type
- Polyclonal
- Antigen
- GST fusion protein corresponding to the carboxy-terminus of Human Cox-2.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 0.1 ml
- Concentration
- 55 mg/ml (by Refractometry)
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Supportive validation
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- Acris Antibodies GmbH (provider)
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- Experimental details
- Western Blot analysis using R1185 Cox2 antibody to detect Cox-2 present in Cox-2 transfected Sf9 cell extract (Lane 1) and IL-1B induced WISH cell extract (Lane 2). Detection occurs using a 1/10,000 dilution of antibody followed by 1/10,000 dilution of HRP Goat anti-Rabbit with visualization via ECL. Film exposure approximately 1'. Other detection systems will yield similar results.