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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Western blot [1]
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- Product number
- NB600-589 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB600-589, RRID:AB_10002874
- Product name
- Rabbit Polyclonal Arginase 1/ARG1/liver Arginase Antibody
- Antibody type
- Polyclonal
- Description
- Delipidation and Defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Arginase [Bovine Liver].
- Reactivity
- Bovine
- Host
- Rabbit
- Vial size
- 1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: Arginase 1/ARG1/liver Arginase Antibody [NB600-589] - Lane 1: Arginase. Lane 2: None. Load: 50 ng per lane. Primary antibody: Arginase primary antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Blocking: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 35 kDa, 39 kDa for Arginase. Other band(s): Arginase splice variants and isoforms.
