IC5868F

antibody from Novus Biologicals

Targeting: ARG1

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

IC5868F

Provider

Novus Biologicals

Product name

Sheep Polyclonal Arginase 1/ARG1/liver Arginase Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects human, mouse, and rat Arginase 1/ARG1 in direct ELISAs and Western blots.

Reactivity

Human, Mouse

Host

Sheep

Isotype

IgG

Vial size

100 Tests

Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied.

References

Submitted references

Hippocampal neural stem cells and microglia response to experimental inflammatory bowel disease (IBD).

Gampierakis IA, Koutmani Y, Semitekolou M, Morianos I, Charalampopoulos I, Xanthou G, Gravanis A, Karalis KP
Molecular psychiatry 2020 Jan 22;

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Astragalus Polysaccharide RAP Induces Macrophage Phenotype Polarization to M1 via the Notch Signaling Pathway.

Wei W, Li ZP, Bian ZX, Han QB
Molecules (Basel, Switzerland) 2019 May 27;24(10)

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Systemic T Cells Immunosuppression of Glioma Stem Cell-Derived Exosomes Is Mediated by Monocytic Myeloid-Derived Suppressor Cells.

Domenis R, Cesselli D, Toffoletto B, Bourkoula E, Caponnetto F, Manini I, Beltrami AP, Ius T, Skrap M, Di Loreto C, Gri G
PloS one 2017;12(1):e0169932

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Phosphorylated exogenous alpha-synuclein fibrils exacerbate pathology and induce neuronal dysfunction in mice.

Karampetsou M, Ardah MT, Semitekolou M, Polissidis A, Samiotaki M, Kalomoiri M, Majbour N, Xanthou G, El-Agnaf OMA, Vekrellis K
Scientific reports 2017 Nov 28;7(1):16533

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Modulation of Microglial Cell Fcγ Receptor Expression Following Viral Brain Infection.

Chauhan P, Hu S, Sheng WS, Prasad S, Lokensgard JR
Scientific reports 2017 Feb 6;7:41889

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NK Cells Alleviate Lung Inflammation by Negatively Regulating Group 2 Innate Lymphoid Cells.

Bi J, Cui L, Yu G, Yang X, Chen Y, Wan X
Journal of immunology (Baltimore, Md. : 1950) 2017 Apr 15;198(8):3336-3344

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Targeting Ornithine Decarboxylase by α-Difluoromethylornithine Inhibits Tumor Growth by Impairing Myeloid-Derived Suppressor Cells.

Ye C, Geng Z, Dominguez D, Chen S, Fan J, Qin L, Long A, Zhang Y, Kuzel TM, Zhang B
Journal of immunology (Baltimore, Md. : 1950) 2016 Jan 15;196(2):915-23

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Increased levels of granulocytic myeloid-derived suppressor cells in peripheral blood and tumour tissue of pancreatic cancer patients.

Khaled YS, Ammori BJ, Elkord E
Journal of immunology research 2014;2014:879897

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Immunosuppressive CD71+ erythroid cells compromise neonatal host defence against infection.

Elahi S, Ertelt JM, Kinder JM, Jiang TT, Zhang X, Xin L, Chaturvedi V, Strong BS, Qualls JE, Steinbrecher KA, Kalfa TA, Shaaban AF, Way SS
Nature 2013 Dec 5;504(7478):158-62

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Myeloid-derived suppressor cell measurements in fresh and cryopreserved blood samples.

Kotsakis A, Harasymczuk M, Schilling B, Georgoulias V, Argiris A, Whiteside TL
Journal of immunological methods 2012 Jul 31;381(1-2):14-22

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Arginase 1/ARG1 in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Sheep Anti-Human/Mouse Arginase 1/ARG1 Fluorescein-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC5868F, filled histogram) or isotype control antibody (Catalog # IC016F, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Arginase 1/ARG1 in Hepa 1-6 Mouse Cell Line by Flow Cytometry. Hepa 1-6 mouse hepatoma cell line was stained with Sheep Anti-Human/Mouse Arginase 1/ARG1 Fluorescein-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC5868F, filled histogram) or isotype control antibody (Catalog # IC016F, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.