Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- 678802 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#678802, RRID:AB_2566051
- Product name
- Purified anti-Arginase I
- Antibody type
- Monoclonal
- Antigen
- Full length recombinant human ARG1 protein produced in HEK293T cells.
- Description
- ARG1 (Arginase 1), is a marker for the M2 anti-inflammatory macrophage subset that hydrolyzes l-arginine into urea and ornithine, and plays an important role in wound healing, antiviral immune responses, and atherosclerosis. The inherited deficiency of this enzyme results in argininemia, which is an autosomal recessive disorder characterized by hyperammonemia. Recent studies show that intracellular pathogens induce the expression of ARG1 in mouse macrophages through the Toll-like receptor (TLR) pathway. The deletion of this enzyme in macrophages favors host survival. Other studies indicate that ARG1 also plays a role in T cell responses and selective antagonists of ARG1 might prove beneficial in fighting diseases in which T-cell responses are inappropriately suppressed.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- O94E6/ARG1
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- Western blot analysis of cell lysates from Jurkat (lane 1), PBMC (lane 2), and THP-1 untreated (lane 3). THP-1 was treated with PMA 100ng/ml for three days, then LPS 1 µg/ml for 24 hours (lane 4), mouse liver (lane 5), MCF-7 (lane 6), and HepG2 (lane 7). Anti-ARG1 antibody (clone O94E6/ARG1 ) was used and followed by HRP anti-mouse IgG antibody. β-actin antibody (clone poly6221) or GAPDH (clone poly6314) were used as loading controls.
- Conjugate
- Unconjugated