50-9779-80

antibody from Invitrogen Antibodies

Targeting: ARG1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

50-9779-80

Provider

Invitrogen Antibodies

Product name

Arginase 1 Monoclonal Antibody (sl6arg), eFluor™ 660, eBioscience™

Antibody type

Monoclonal

Antigen

Other

Description

Description: The monoclonal antibody sl6arg recognizes human Arginase-1, a 35 kDa enzyme that that helps eliminate nitrogen by converting L-arginine to urea and L-ornithine which further converts to polyamine. These polyamines are important for cell proliferation and removal of toxins that arise from protein degradation. Expression is found in the liver, neutrophils, as well as myeloid derived suppressor cells (MDSC) and neural stem cells. Expression in macrophages has not been determined in humans, while in mice expression in M2 macrophages is well established. Arginase-1 may be expressed in the myeloid population in breast cancer tumors and is typically found in the majority of hepatocellular carcinomas.

Antibody clone number

sl6arg

Concentration

0.2 mg/mL

References

Submitted references

Pulmonary Conventional Type 1 Langerin-Expressing Dendritic Cells Play a Role in Impairing Early Protective Immune Response against Cryptococcus neoformans Infection in Mice.

Guasconi L, Beccacece I, Volpini X, Burstein VL, Mena CJ, Silvane L, Almeida MA, Musri MM, Cervi L, Chiapello LS
Journal of fungi (Basel, Switzerland) 2022 Jul 28;8(8)

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-Arginase 1 Monoclonal Antibody (sl6arg), eFluor 660, eBioscience™ (Product # 50-9779-82) and a 37 kDa band corresponding to Arginase-1 was observed. Whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), MOLT-4 (Lane 2), DU 145 (Lane 3) and LNCaP (Lane 4) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:500) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076). Relative expression was observed between DU145 and LNCaP (doi: 10.1371/journal.pone.0012107).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of formalin-fixed paraffin embedded human liver tissue using 5 µg/mL of Mouse IgG1 K Isotype Control eFluor® 660 (left) or 5 µg/mL of Anti-Human Arginase-1 eFluor® 660 (right). Nuclei are stained with DAPI.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Ex vivo cytokine production, fungal growth inhibition and arginase-1 or iNOS expression by lung cells from C. neoformans -infected mice. ( A ) Cytokine production by lung cell suspensions obtained from DT-treated (black bars) or untreated (white bars) LangDTREGFP mice at 7 days post infection. Bars represent cytokine levels (ELISA) in 24 h culture supernatants. * p < 0.05; ** p < 0.005. ( B ) In vitro C. neoformans growth assay using a yeast suspension (1 x 10 3 ) cultured in medium alone (gray bar) or in the presence of adherent lung cells from untreated (white bar) or DT-treated (black bar) 7-day-infected LangDTREGFP mice. Bars represent ratio of final vs initial CFU numbers in each culture condition. ( C ) Arginase activity from lysates of adherent cells cultured in ( B ), measured as urea production (ug of urea per ug of protein). * p < 0.05. Data are expressed as mean +- SEM. The data shown are pooled from two independent experiments ( n = 6 animals per group; samples from each animal were analyzed in triplicate ( A , C ) or duplicate ( B )). All data were analyzed with Student's t -test or ANOVA. ( D ) Confocal microscopy of representative lung sections from 7-day-infected mice ( n = 6) after immunostaining with fluorochrome-labeled specific antibodies to arginase-1 (upper panels) or iNOS (lower panels). 600x magnification. DT: diphtheria toxin; dpi: days post infection; CFU: colony-forming units.