Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Other assay [1]
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- Product number
- 53-9779-80 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Arginase 1 Monoclonal Antibody (sl6arg), Alexa Fluor™ 488, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The monoclonal antibody sl6arg recognizes human Arginase-1, a 35 kDa enzyme that that helps eliminate nitrogen by converting L-arginine to urea and L-ornithine which further converts to polyamine. These polyamines are important for cell proliferation and removal of toxins that arise from protein degradation. Expression is found in the liver, neutrophils, as well as myeloid derived suppressor cells (MDSC) and neural stem cells. Expression in macrophages has not been determined in humans, while in mice expression in M2 macrophages is well established. Arginase-1 may be expressed in the myeloid population in breast cancer tumors and is typically found in the majority of hepatocellular carcinomas.
- Conjugate
- Green dye
- Antibody clone number
- sl6arg
- Concentration
- 0.5 mg/mL
Submitted references Pulmonary Conventional Type 1 Langerin-Expressing Dendritic Cells Play a Role in Impairing Early Protective Immune Response against Cryptococcus neoformans Infection in Mice.
Guasconi L, Beccacece I, Volpini X, Burstein VL, Mena CJ, Silvane L, Almeida MA, Musri MM, Cervi L, Chiapello LS
Journal of fungi (Basel, Switzerland) 2022 Jul 28;8(8)
Journal of fungi (Basel, Switzerland) 2022 Jul 28;8(8)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry of fixed and permeabilized HepG2 cells stained with 20 µg/mL Mouse IgG1 K Isotype Control Alexa Fluor® 488 (left) or 20 µg/mL Anti-Human Arginase-1 Alexa Fluor® 488 (right). Nuclei are stained with DAPI.
- Conjugate
- Green dye
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Ex vivo cytokine production, fungal growth inhibition and arginase-1 or iNOS expression by lung cells from C. neoformans -infected mice. ( A ) Cytokine production by lung cell suspensions obtained from DT-treated (black bars) or untreated (white bars) LangDTREGFP mice at 7 days post infection. Bars represent cytokine levels (ELISA) in 24 h culture supernatants. * p < 0.05; ** p < 0.005. ( B ) In vitro C. neoformans growth assay using a yeast suspension (1 x 10 3 ) cultured in medium alone (gray bar) or in the presence of adherent lung cells from untreated (white bar) or DT-treated (black bar) 7-day-infected LangDTREGFP mice. Bars represent ratio of final vs initial CFU numbers in each culture condition. ( C ) Arginase activity from lysates of adherent cells cultured in ( B ), measured as urea production (ug of urea per ug of protein). * p < 0.05. Data are expressed as mean +- SEM. The data shown are pooled from two independent experiments ( n = 6 animals per group; samples from each animal were analyzed in triplicate ( A , C ) or duplicate ( B )). All data were analyzed with Student's t -test or ANOVA. ( D ) Confocal microscopy of representative lung sections from 7-day-infected mice ( n = 6) after immunostaining with fluorochrome-labeled specific antibodies to arginase-1 (upper panels) or iNOS (lower panels). 600x magnification. DT: diphtheria toxin; dpi: days post infection; CFU: colony-forming units.
- Conjugate
- Green dye