Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- IC5868N-100UG - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse Arginase 1/ARG1 Alexa Fluor® 700-conjugated Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human, mouse, and rat Arginase 1/ARG1 in direct ELISAs and Western blots.
- Reactivity
- Human, Mouse
- Host
- Sheep
- Conjugate
- Near infrared dye
- Antigen sequence
P05089
- Isotype
- IgG
- Vial size
- 100 ug
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Arginase 1/ARG1 in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Sheep Anti-Human/Mouse Arginase 1/ARG1 Alexa Fluor® 700-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC5868N, filled histogram) or isotype control antibody (Catalog # IC016N, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Membrane-associated Proteins.