PA5-85267

antibody from Invitrogen Antibodies

Targeting: ARG1

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-85267

Provider

Invitrogen Antibodies

Product name

Arginase 1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Keep as concentrated solution. Predicted reactivity: Mouse (86%), Rat (86%), Pig (89%), Rabbit (91%), Bovine (90%). Positive Control: Huh7, HepG2, mouse liver, rat liver. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.72 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

HA-coated collagen nanofibers for urethral regeneration via in situ polarization of M2 macrophages.

Niu Y, Stadler FJ, Yang X, Deng F, Liu G, Xia H
Journal of nanobiotechnology 2021 Sep 22;19(1):283

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DA-DRD5 signaling controls colitis by regulating colonic M1/M2 macrophage polarization.

Liu L, Wu Y, Wang B, Jiang Y, Lin L, Li X, Yang S
Cell death & disease 2021 May 17;12(6):500

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Clusterin regulates macrophage expansion, polarization and phagocytic activity in response to inflammation in the kidneys.

Weng X, Zhao H, Guan Q, Shi G, Feng S, Gleave ME, Nguan CC, Du C
Immunology and cell biology 2021 Mar;99(3):274-287

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of Arginase 1 in 4% paraformaldehyde-fixed HeLa cells using Arginase 1 polyclonal antibody (Product # PA5-85267) at a dilution of 1:200.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of Arginase 1 in HepG2 cells (control: unlabeled cells) using Arginase 1 polyclonal antibody (Product # PA5-85267) at a dilution of 1:50. Dylight 488-conjugated was used as a secondary antibody.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 6 CLU expression is associated with decreased ratio of NOS 2 + (M1 marker)/ARG 1 + (M2 marker) cells at day 7 following renal IRI. ARG 1 + and NOS 2 + cells were identified by immunohistochemistry as described in the ""Methods"" section. (a) The numbers of ARG 1 + cells. * P = 0.0010 (WT versus KO, two-tailed t -test). (b) The numbers of NOS 2 + cells. ** P = 0.0021 (WT versus KO, two-tailed t -test). (c) The difference in the M1/M2 ratio between the WT and CLU-KO groups was confirmed by the NOS 2 + /ARG 1 + ratio in each kidney. *** P = 0.0179 (WT versus KO, two-tailed t -test). Data in a , b and c are representative of three independent experiments and presented as the mean +- s.d. ( n = 3). (d) Representative images of three independent experiments. ARG 1 + (top 2) and NOS 2 + (bottom 2) cells (dark brown) in the tubulointerstitial area, the perivascular space and injured tubular epithelium of the renal cortical and medullary junction. Red arrowheads represent positively stained cells; Black arrowheads correspond to false-positive staining. Scale bar: 100 um. ARG 1; arginase 1; CLU, clusterin; DT, distal convoluted tubule; G, glomerulus; IA, interlobular artery; KO, clusterin knockout; NOS 2, nitric oxide synthase 2; PT, proximal convoluted tubule; WT, wild type.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation analysis of Arginase 1 in HepG2 whole cell extracts with Arginase 1 polyclonal antibody (Product # PA5-85267) using 5 µg of sample, followed by anti-Rabbit IgG secondary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 5 The deficiency of DRD5 signaling increased colonic M1 macrophages but reduced M2 cells in the colon of DSS colitis mice. A Immunofluorescent labeling of F4/80 + , Arg1 + , and Inos + colonic macrophages of WT and DRD5 -/- mice on day 6 after DSS treatment (top). The cell numbers are quantified by Image pro plus (bottom) ( n = 3). Scale bars, 200 mum. B Flow cytometry analysis of colonic macrophages of WT and DRD5 -/- mice ( n = 5 mice per group) on day 6 after DSS treatment as indicated. Data are presented as representative plots (top) and summary graphs of quantified percentages (bottom). C Flow cytometry analysis of the CD45.2 + cells percentage in Arg1 + and CD86 + colonic macrophages of lethally irradiated mice reconstituted with DRD5 -/- CD45.2/WT CD45.1 bone marrow (1:1 ratio), and a control WT CD45.2/WT CD45.1 group (1:1 ratio) on day 6 after DSS treatment ( n = 3 mice per group). Data are presented as representative plots (left) and summary graphs of quantified percentages (right). Data are pooled from two or three independent experiments. Error bars show means +- SEM. * p < 0.05, **p < 0.01, *** p < 0.001. Two-tailed unpaired student's t -test ( A , B , C ).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 6 CLU expression is associated with decreased ratio of NOS 2 + (M1 marker)/ARG 1 + (M2 marker) cells at day 7 following renal IRI. ARG 1 + and NOS 2 + cells were identified by immunohistochemistry as described in the ""Methods"" section. (a) The numbers of ARG 1 + cells. * P = 0.0010 (WT versus KO, two-tailed t -test). (b) The numbers of NOS 2 + cells. ** P = 0.0021 (WT versus KO, two-tailed t -test). (c) The difference in the M1/M2 ratio between the WT and CLU-KO groups was confirmed by the NOS 2 + /ARG 1 + ratio in each kidney. *** P = 0.0179 (WT versus KO, two-tailed t -test). Data in a , b and c are representative of three independent experiments and presented as the mean +- s.d. ( n = 3). (d) Representative images of three independent experiments. ARG 1 + (top 2) and NOS 2 + (bottom 2) cells (dark brown) in the tubulointerstitial area, the perivascular space and injured tubular epithelium of the renal cortical and medullary junction. Red arrowheads represent positively stained cells; Black arrowheads correspond to false-positive staining. Scale bar: 100 um. ARG 1; arginase 1; CLU, clusterin; DT, distal convoluted tubule; G, glomerulus; IA, interlobular artery; KO, clusterin knockout; NOS 2, nitric oxide synthase 2; PT, proximal convoluted tubule; WT, wild type.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 4 The polarization of macrophages to M2 phenotype is related to the elongated cell shape. A Fluorescence micrographs of Raw 264.7 macrophages immune-stained for arginase-1 (green), iNOS (red), and nuclear counterstain (blue) on cell plate (control), collagen and HA-collagen nanofibrous films. Scale bars: 15 mum. B Representative Western blot of arginase-1, iNOS, and tubulin of control, collagen and HA-collagen nanofibrous films and quantification of average across three separate experiments. Quantified TNF-alpha C and IL-10 D secretion from macrophages cultured on different nanofibrous scaffolds or culture plates using ELISA assay. n = 3, ** p < 0.01, *** p < 0.001