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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunohistochemistry [1]
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- Product number
- PA5-32267 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Arginase 1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is hepatocellular carcinoma.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 500 µL
- Concentration
- 0.046 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Knockout of TRPA1 exacerbates angiotensin II-induced kidney injury.
Ma S, Zhang Y, He K, Wang P, Wang DH
American journal of physiology. Renal physiology 2019 Sep 1;317(3):F623-F631
American journal of physiology. Renal physiology 2019 Sep 1;317(3):F623-F631
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunohistochemical (paraffin) analysis of Arginase-1 using anti-Arginase-1 Polyclonal Antibody (Product # PA5-32267) in Hepatocellular Carcinoma Cancer Tissue. The recommended dilution for this antibody in immunohistochemistry applications is 1:100.
