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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [7]
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- Validations
- Flow cytometry [2]
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- Product number
- IC5868A - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse Arginase 1/ARG1 APC-conjugated Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human, mouse, and rat Arginase 1/ARG1 in direct ELISAs and Western blots.
- Reactivity
- Human, Mouse
- Host
- Sheep
- Conjugate
- Red dye
- Antigen sequence
P05089- Isotype
- IgG
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
Submitted references IL-6 mediates ER expansion during hyperpolarization of alternatively activated macrophages.
CCR2-dependent monocytes/macrophages exacerbate acute brain injury but promote functional recovery after ischemic stroke in mice.
Schwann Cell Transplantation Subdues the Pro-Inflammatory Innate Immune Cell Response after Spinal Cord Injury.
Compensatory upregulation of PD-1, LAG-3, and CTLA-4 limits the efficacy of single-agent checkpoint blockade in metastatic ovarian cancer.
Overexpression of OSM and IL-6 impacts the polarization of pro-fibrotic macrophages and the development of bleomycin-induced lung fibrosis.
Deletion of astroglial CXCL10 delays clinical onset but does not affect progressive axon loss in a murine autoimmune multiple sclerosis model.
Conditional ablation of astroglial CCL2 suppresses CNS accumulation of M1 macrophages and preserves axons in mice with MOG peptide EAE.
Ayaub EA, Tandon K, Padwal M, Imani J, Patel H, Dubey A, Mekhael O, Upagupta C, Ayoub A, Dvorkin-Gheva A, Murphy J, Kolb PS, Lhotak S, Dickhout JG, Austin RC, Kolb MRJ, Richards CD, Ask K
Immunology and cell biology 2019 Feb;97(2):203-217
Immunology and cell biology 2019 Feb;97(2):203-217
CCR2-dependent monocytes/macrophages exacerbate acute brain injury but promote functional recovery after ischemic stroke in mice.
Fang W, Zhai X, Han D, Xiong X, Wang T, Zeng X, He S, Liu R, Miyata M, Xu B, Zhao H
Theranostics 2018;8(13):3530-3543
Theranostics 2018;8(13):3530-3543
Schwann Cell Transplantation Subdues the Pro-Inflammatory Innate Immune Cell Response after Spinal Cord Injury.
Pearse DD, Bastidas J, Izabel SS, Ghosh M
International journal of molecular sciences 2018 Aug 28;19(9)
International journal of molecular sciences 2018 Aug 28;19(9)
Compensatory upregulation of PD-1, LAG-3, and CTLA-4 limits the efficacy of single-agent checkpoint blockade in metastatic ovarian cancer.
Huang RY, Francois A, McGray AR, Miliotto A, Odunsi K
Oncoimmunology 2017;6(1):e1249561
Oncoimmunology 2017;6(1):e1249561
Overexpression of OSM and IL-6 impacts the polarization of pro-fibrotic macrophages and the development of bleomycin-induced lung fibrosis.
Ayaub EA, Dubey A, Imani J, Botelho F, Kolb MRJ, Richards CD, Ask K
Scientific reports 2017 Oct 16;7(1):13281
Scientific reports 2017 Oct 16;7(1):13281
Deletion of astroglial CXCL10 delays clinical onset but does not affect progressive axon loss in a murine autoimmune multiple sclerosis model.
Mills Ko E, Ma JH, Guo F, Miers L, Lee E, Bannerman P, Burns T, Ko D, Sohn J, Soulika AM, Pleasure D
Journal of neuroinflammation 2014 Jun 12;11:105
Journal of neuroinflammation 2014 Jun 12;11:105
Conditional ablation of astroglial CCL2 suppresses CNS accumulation of M1 macrophages and preserves axons in mice with MOG peptide EAE.
Moreno M, Bannerman P, Ma J, Guo F, Miers L, Soulika AM, Pleasure D
The Journal of neuroscience : the official journal of the Society for Neuroscience 2014 Jun 11;34(24):8175-85
The Journal of neuroscience : the official journal of the Society for Neuroscience 2014 Jun 11;34(24):8175-85
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Arginase 1/ARG1 in Hepa 1-6 Mouse Cell Line by Flow Cytometry. Hepa 1-6 mouse hepatoma cell line was stained with Sheep Anti-Human/Mouse Arginase 1/ARG1 APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC5868A, filled histogram) or isotype control antibody (Catalog # IC016A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Arginase 1/ARG1 in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Sheep Anti-Human/Mouse Arginase 1/ARG1 APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC5868A, filled histogram) or isotype control antibody (Catalog # IC016A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
