PA5-80139

antibody from Invitrogen Antibodies

Targeting: TMEM240 C1orf70, SCA21

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-80139

Provider

Invitrogen Antibodies

Product name

TMEM240 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL. Positive Control - WB: rat brain tissue, NIH3T3 whole cell, COLO320 whole cell.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

500 µg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of TMEM240 in, Lane 1: rat brain tissue lysates, Lane 2: NIH3T3 whole Cell lysates, Lane 3: COLO320 whole cell lysates. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 µg of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. The membrane was blocked with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with TMEM240 Polyclonal Antibody (Product # PA5-80139) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0. 1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit. A specific band was detected for TMEM240 at approximately 19KD. The expected band size for TMEM240 is at 19KD.