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- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- PA5-22121 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PTS Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Recommended positive controls: 293T, H1299, HeLa, mouse stomach, PTS-transfected 293T.
- Concentration
- 1 mg/mL
Submitted references High-grade extracellular vesicles preparation by combined size-exclusion and affinity chromatography.
Bellotti C, Lang K, Kuplennik N, Sosnik A, Steinfeld R
Scientific reports 2021 May 18;11(1):10550
Scientific reports 2021 May 18;11(1):10550
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of PTS was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 15% SDS-PAGE. Proteins were transferred to a membrane and probed with a PTS Polyclonal Antibody (Product # PA5-22121) at a dilution of 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using PTS Polyclonal Antibody (Product # PA5-22121). Sample (50 µg of whole cell lysate). Lane A: mouse stomach . 15% SDS PAGE. PTS Polyclonal Antibody (Product # PA5-22121) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using PTS Polyclonal Antibody (Product # PA5-22121). Sample (30 µg of whole cell lysate). Lane A: H1299 .15% SDS PAGE. PTS Polyclonal Antibody (Product # PA5-22121) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of PTS in methanol-fixed HeLa cells using a PTS polyclonal antibody (Product # PA5-22121) at a 1:100 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of methanol-fixed HeLa, using PTS antibody (Product # PA5-22121) at 1:100 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human hepatoma, using PTS (Product # PA5-22121) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3 Characterization of EVs obtained by FPLC in comparison to EVs isolated by the DC protocol. EVs were prepared as described for the specific method. One sample from HEK293 cells expressing polyhistidine-tagged FRalpha was split into two halves to compare the expression of protein markers. For each step and method 1 ug total protein was loaded onto a gel. The blots were probed for EV-enriched proteins like FRalpha, flotillin and Alix. Additionally, the reduction of intra- and extracellular contaminations was investigated with the proteins TOM20 and the stably expressed PTPS. For this analysis we considered also the intermediate centrifugation steps of the DC protocol. Peak II (PII) of the two-step FPLC combining SEC and IMAC shows the strongest recovery of EV markers and reduction in EV-negative ones.
