AHR-004-25UL

antibody from Invitrogen Antibodies

Targeting: HRH4 AXOR35, GPCR105, GPRv53, H4R, HH4R

Western blot (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

AHR-004-25UL

Provider

Invitrogen Antibodies

Product name

Histamine H4 Receptor (HRH4) (extracellular) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

25 µL

Concentration

0.7 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysisof human chronic myelogenous leukemia (K562) (lanes 1 and 3) and human promyelocytic leukemia (HL-60) (lanes 2 and 4) cell lysates: - 1,2. Anti-Human Histamine H4Receptor (HRH4) (extracellular) Antibody (#AHR-004), (1:400).3,4. Anti-Human Histamine H4Receptor (HRH4) (extracellular) Antibody , preincubated with Human Histamine H4Receptor/HRH4 (extracellular) Blocking Peptide (#BLP-HR004).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysisof human chronic myelogenous leukemia (K562) (lanes 1 and 3) and human promyelocytic leukemia (HL-60) (lanes 2 and 4) cell lysates: - 1,2. Anti-Human Histamine H4Receptor (HRH4) (extracellular) Antibody (#AHR-004), (1:400).3,4. Anti-Human Histamine H4Receptor (HRH4) (extracellular) Antibody , preincubated with Human Histamine H4Receptor/HRH4 (extracellular) Blocking Peptide (#BLP-HR004).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Cell surface detection of Histamine H4 receptor by indirect flow cytometry in live intact human HL-60 cell leukemia cells: - (black line) cells. (red) Cells + goat- Anti-rabbit-FITC. (green) Cells + Anti-Human Histamine H4 Receptor (HRH4) (extracellular) Antibody (#AHR-004), (5μg) + goat- Anti-rabbit-FITC.