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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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- Product number
- H00004950-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00004950-M01, RRID:AB_546651
- Product name
- Mouse Monoclonal Occludin Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. OCLN - occludin
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Development of a Perfusion Platform for Dynamic Cultivation of in vitro Skin Models.
Endoplasmic reticulum protein 29 regulates epithelial cell integrity during the mesenchymal-epithelial transition in breast cancer cells.
Strüver K, Friess W, Hedtrich S
Skin pharmacology and physiology 2017;30(4):180-189
Skin pharmacology and physiology 2017;30(4):180-189
Endoplasmic reticulum protein 29 regulates epithelial cell integrity during the mesenchymal-epithelial transition in breast cancer cells.
Bambang IF, Lee YK, Richardson DR, Zhang D
Oncogene 2013 Mar 7;32(10):1240-51
Oncogene 2013 Mar 7;32(10):1240-51
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Occludin Antibody (1G7) [H00004950-M01] - OCLN monoclonal antibody (M01), clone 1G7 Analysis of OCLN expression in HL-60.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: Occludin Antibody (1G7) [H00004950-M01] - Detection limit for recombinant GST tagged OCLN is approximately 0.1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: Occludin Antibody (1G7) [H00004950-M01] - Analysis of monoclonal antibody to OCLN on HeLa cell. Antibody concentration 10 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: Occludin Antibody (1G7) [H00004950-M01] - Analysis of OCLN transfected lysate using anti-OCLN monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with OCLN MaxPab rabbit polyclonal antibody.
