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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [6]
- Other assay [3]
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- Product number
- PA5-20755 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Occludin Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- A suggested positive control is human liver tissue lysate.
- Concentration
- 1 mg/mL
Submitted references Curcumin reduces blood-nerve barrier abnormalities and cytotoxicity to endothelial cells and pericytes induced by cisplatin.
Lycium barbarum Polysaccharide Ameliorates Heat-Stress-Induced Impairment of Primary Sertoli Cells and the Blood-Testis Barrier in Rat via Androgen Receptor and Akt Phosphorylation.
Blood-Brain Barrier Permeability: Is 5-Hydroxytryptamine Receptor Type 4 a Game Changer?
Transcriptomic profiles reveal differences in zinc metabolism, inflammation, and tight junction proteins in duodenum from cholesterol gallstone subjects.
Kobutree P, Tothonglor A, Roumwong A, Jindatip D, Agthong S
Folia morphologica 2022 Jul 12;
Folia morphologica 2022 Jul 12;
Lycium barbarum Polysaccharide Ameliorates Heat-Stress-Induced Impairment of Primary Sertoli Cells and the Blood-Testis Barrier in Rat via Androgen Receptor and Akt Phosphorylation.
Hu S, Liu D, Liu S, Li C, Guo J
Evidence-based complementary and alternative medicine : eCAM 2021;2021:5574202
Evidence-based complementary and alternative medicine : eCAM 2021;2021:5574202
Blood-Brain Barrier Permeability: Is 5-Hydroxytryptamine Receptor Type 4 a Game Changer?
Becker G, Da Silva S, Sabo AN, Antal MC, Kemmel V, Monassier L
Pharmaceutics 2021 Nov 3;13(11)
Pharmaceutics 2021 Nov 3;13(11)
Transcriptomic profiles reveal differences in zinc metabolism, inflammation, and tight junction proteins in duodenum from cholesterol gallstone subjects.
Riveras E, Azocar L, Moyano TC, Ocares M, Molina H, Romero D, Roa JC, Valbuena JR, Gutiérrez RA, Miquel JF
Scientific reports 2020 May 4;10(1):7448
Scientific reports 2020 May 4;10(1):7448
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human liver tissue lysate using a OCLN polyclonal antibody (Product # PA5-20755) at 1 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of OCLN in human liver tissue lysate with Occludin Polyclonal Antibody (Product # PA5-20755) at 1 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of human liver cells using a OCLN polyclonal antibody (Product # PA5-20755) at a 20 µg/mL dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of OCLN in mouse brain tissue with Occludin Polyclonal Antibody (Product # PA5-20755) at 20 µg/mL. Red: OCLN Blue: DAPI staining
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of OCLN in Human Liver tissue with Occludin Polyclonal Antibody (Product # PA5-20755) at 20 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of OCLN in human liver tissue with Occludin Polyclonal Antibody (Product # PA5-20755) at 20 µg/mL. Red: OCLN Blue: DAPI staining
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of OCLN in mouse brain tissue with Occludin Polyclonal Antibody (Product # PA5-20755) at 20 µg/mL. Red: OCLN Blue: DAPI staining
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of OCLN in human liver tissue with Occludin Polyclonal Antibody (Product # PA5-20755) at 2.5 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of OCLN in mouse brain tissue with Occludin Polyclonal Antibody (Product # PA5-20755) at 5 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4 Tight junction gene expressions are decreased on duodenal biopsy of cholesterol gallstone patients. Gene expression, immunofluorescence and densitometry analysis of TJP1 (left) and OCLN (right) of biopsied duodenal tissue: A) TJP1 and OCLN gene expression of healthy volunteers (n = 24, white bars) and GSD patients (N = 24, black bars) was evaluated by qPCR. B) TJP1 and OCLN protein expression were quantified in formalin-fixed paraffin-embedded tissue samples from duodenal mucosa of GSD and control subjects by measuring the average fluorescent intensity in 10 non-overlapping fields per subject by microscopy. Data are the mean of three control subjects and six patients with GSD. ***p < 0.001.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 5 LBP maintains the integrity of BTB after heat stress. Occludin and ZO-1 in SCs were detected with western blot (a, b). Data are presented as means +- SEM ( n = 3) from three independent experiments. ** p < 0.01, compared to the control group. # p < 0.05, ## p < 0.01, compared to the heat-stress group.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 6 Western blot analysis showing the involvement of the 5-HT 4 receptor in the regulation of the expression of tight junction proteins in hCMEC/D3 cells. Cells were treated with GR113808 (1 uM), prucalopride (10 uM) or GR113808 (1 uM) + prucalopride (10 uM) for 96 h. Relative integrated density values of ( a ) ZO-1, ( b ) claudin-5 and ( c ) occludin are shown. No correlation is found between the treatment and the expression of ZO-1 and claudin-5. A significant negative correlation between treatment with prucalopride and the expression of occludin is apparent, compared to control. Histograms represent mean +- SEM from three independent experiments. * p < 0.05.
