LS-B11010

antibody from LSBio

Targeting: TJP1 DKFZp686M05161, MGC133289, ZO-1

Western blot (Enhanced validation)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

LS-B11010

Provider

LSBio

Product name

PathPlus™ TJP1 / ZO-1 Antibody (aa1-296) LS-B11010

Antibody type

Polyclonal

Description

Immunoaffinity purified

Reactivity

Human

Host

Rabbit

Isotype

IgG

Storage

Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Sample (30 ug whole cell lysate). A: H1299. 5% SDS PAGE. ZO-1 antibody diluted at 1:3000

Immunocytochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

ZO-1 antibody, N-term detects TJP1 protein at junction by confocal immunofluorescent analysis. A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. TJP1 protein stained by ZO-1 antibody, N-term diluted at 1:500.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

ZO-1 antibody, N-term detects TJP1 protein at junction by confocal immunofluorescent analysis. A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. TJP1 protein stained by ZO-1 antibody, N-term diluted at 1:500.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

ZO-1 antibody, N-term detects TJP1 protein at junction by confocal immunofluorescent analysis. A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. TJP1 protein stained by ZO-1 antibody, N-term diluted at 1:500.