37-4700

antibody from Invitrogen Antibodies

Targeting: TJP2 DFNA51, X104, ZO-2, ZO2

Western blot (Recommended by provider)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

37-4700

Provider

Invitrogen Antibodies

Product name

ZO-2 Monoclonal Antibody (3E8D9)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Reactivity

Human, Canine

Host

Mouse

Isotype

IgG

Antibody clone number

3E8D9

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

-20°C

References

Submitted references

PAI-1-Dependent Inactivation of SMAD4-Modulated Junction and Adhesion Complex in Obese Endometrial Cancer.

Lin LL, Kost ER, Lin CL, Valente P, Wang CM, Kolonin MG, Daquinag AC, Tan X, Lucio N, Hung CN, Wang CP, Kirma NB, Huang TH
Cell reports 2020 Oct 13;33(2):108253

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The tight junction protein TJP1 regulates the feeding-modulated hepatic circadian clock.

Liu Y, Zhang Y, Li T, Han J, Wang Y
Nature communications 2020 Jan 30;11(1):589

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Entamoeba histolytica EhCP112 Dislocates and Degrades Claudin-1 and Claudin-2 at Tight Junctions of the Intestinal Epithelium.

Cuellar P, Hernández-Nava E, García-Rivera G, Chávez-Munguía B, Schnoor M, Betanzos A, Orozco E
Frontiers in cellular and infection microbiology 2017;7:372

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p38δ mitogen-activated protein kinase regulates the expression of tight junction protein ZO-1 in differentiating human epidermal keratinocytes.

Siljamäki E, Raiko L, Toriseva M, Nissinen L, Näreoja T, Peltonen J, Kähäri VM, Peltonen S
Archives of dermatological research 2014 Mar;306(2):131-41

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ZO proteins redundantly regulate the transcription factor DbpA/ZONAB.

Spadaro D, Tapia R, Jond L, Sudol M, Fanning AS, Citi S
The Journal of biological chemistry 2014 Aug 8;289(32):22500-11

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12/15-Lipoxygenase mediates high-fat diet-induced endothelial tight junction disruption and monocyte transmigration: a new role for 15(S)-hydroxyeicosatetraenoic acid in endothelial cell dysfunction.

Kundumani-Sridharan V, Dyukova E, Hansen DE 3rd, Rao GN
The Journal of biological chemistry 2013 May 31;288(22):15830-42

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Label-free analysis of breast tissue polarity by Raman imaging of lipid phase.

Yue S, Cárdenas-Mora JM, Chaboub LS, Lelièvre SA, Cheng JX
Biophysical journal 2012 Mar 7;102(5):1215-23

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Effect of cellular senescence on the albumin permeability of blood-derived endothelial cells.

Cheung TM, Ganatra MP, Peters EB, Truskey GA
American journal of physiology. Heart and circulatory physiology 2012 Dec 1;303(11):H1374-83

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Gene expression profiling of human prostate cancer stem cells reveals a pro-inflammatory phenotype and the importance of extracellular matrix interactions.

Birnie R, Bryce SD, Roome C, Dussupt V, Droop A, Lang SH, Berry PA, Hyde CF, Lewis JL, Stower MJ, Maitland NJ, Collins AT
Genome biology 2008;9(5):R83

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An immunochemical marker for goldfish Mauthner cells.

Flores CE, Ene S, Pereda AE
Journal of neuroscience methods 2008 Oct 30;175(1):64-9

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Cyclin D1 is transcriptionally down-regulated by ZO-2 via an E box and the transcription factor c-Myc.

Huerta M, Muñoz R, Tapia R, Soto-Reyes E, Ramírez L, Recillas-Targa F, González-Mariscal L, López-Bayghen E
Molecular biology of the cell 2007 Dec;18(12):4826-36

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ZO-2/TJP2 Antibody (3E8D9) was done on 90% confluent log phase CACO2 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with ZO-2/TJP2 Antibody (3E8D9) (Product # 37-4700) at 1µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor 488 Rabbit Anti-Mouse IgG Secondary Antibody (Product # A-11059) at a dilution of 1:400 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor 594 Phalloidin (Product # A12381). Panel d is a merged image showing cell junctional localization. Panel e is a no primary antibody control. The images were captured at 40X magnification.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

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Experimental details

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Supportive validation

Submitted by

Invitrogen Antibodies (provider)

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Supportive validation

Submitted by

Invitrogen Antibodies (provider)

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Supportive validation

Submitted by

Invitrogen Antibodies (provider)

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Supportive validation

Submitted by

Invitrogen Antibodies (provider)

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Supportive validation

Submitted by

Invitrogen Antibodies (provider)

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Supportive validation

Submitted by

Invitrogen Antibodies (provider)

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Supportive validation

Submitted by

Invitrogen Antibodies (provider)

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Supportive validation

Submitted by

Invitrogen Antibodies (provider)

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Supportive validation

Submitted by

Invitrogen Antibodies (provider)

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Experimental details

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Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 8 rEhCP112p binds to claudin-1 and claudin-2. Confluent Caco-2 cell monolayers were incubated for 10 min with rEhCP112p, then, cell lysates were immunoprecipitated (IP) in the presence of protease inhibitors, using alpha-EhCP112 antibody or pre-immune serum (PS). Immunoprecipitates, rEhCP112p and Caco-2 cell lysates (Caco-2) were submitted to WB assays and revealed using the antibodies indicated at left.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

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Supportive validation

Submitted by

Invitrogen Antibodies (provider)

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Experimental details

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Supportive validation

Submitted by

Invitrogen Antibodies (provider)

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Experimental details

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Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 7 rEhCP112a degrades claudin-1 and increases claudin-2 expression in Caco-2 cells. (A) Caco-2 cells incubated with rEhCP112a for different times or with live trophozoites (T) for 10 min, submitted to WB using antibodies against TJ proteins indicated at left and alpha-tubulin as a loading control. (B) Densitometry analysis of bands obtained in (A) for each TJs protein with regards to tubulin. * p

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 8 rEhCP112p binds to claudin-1 and claudin-2. Confluent Caco-2 cell monolayers were incubated for 10 min with rEhCP112p, then, cell lysates were immunoprecipitated (IP) in the presence of protease inhibitors, using alpha-EhCP112 antibody or pre-immune serum (PS). Immunoprecipitates, rEhCP112p and Caco-2 cell lysates (Caco-2) were submitted to WB assays and revealed using the antibodies indicated at left.