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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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- Product number
- R31077 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- TJP2 Antibody / ZO-2
- Antibody type
- Polyclonal
- Description
- This highly specific TJP2 antibody is suitable for use in Western blot/Immunohistochemistry/Immunofluorescence applications with human, mouse and rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the TJP2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of TJP2 antibody and Lane 1: rat brain; 2: (r) PC-12; 3: mouse brain; 4: (m) HEPA cell lysate. Predicted/observed size ~131KD
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human RT4, 2) human A431, 3) human HepG2, 4) human MCF7, 5) human U-2 OS, 6) human HeLa, 7) human PC-3, 8) rat RH35 and 9) mouse HEPA1-6 cell lysate with TJP2 antibody. Expected molecular weight: 131-160 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of FFPE human U-2 OS cells with TJP2 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC-P: TJP2 antibody testing of mouse liver tissue
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE mouse liver tissue with TJP2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE rat liver tissue with TJP2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
