PA5-17155

antibody from Invitrogen Antibodies

Targeting: TJP2 DFNA51, X104, ZO-2, ZO2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-17155

Provider

Invitrogen Antibodies

Product name

ZO-2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

It is not recommended to aliquot this antibody. This antibody is not cross-reactive with ZO-1 or ZO-3.

Reactivity

Human, Mouse, Rat, Bovine, Canine

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

44 µg/mL

Storage

-20°C

References

Submitted references

Curcumin reduces blood-nerve barrier abnormalities and cytotoxicity to endothelial cells and pericytes induced by cisplatin.

Kobutree P, Tothonglor A, Roumwong A, Jindatip D, Agthong S
Folia morphologica 2022 Jul 12;

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on membrane enriched extracts (30 µg lysate) of NIH/3T3 (Lane 1), C2C12 (Lane 2) and tissue extract of Mouse Skeletal Muscle (Lane 3). The blot was probed with Anti-ZO-2 Polyclonal Antibody (Product # PA5-17155, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 160 kDa band corresponding to ZO-2 was observed across the cell lines and tissue tested.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of ZO-2 in extracts from C6 and PC-12 cells using ZO-2 polyclonal antibody (Product # PA5-17155).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Knockdown of ZO-2 was achieved by transfecting NIH/3T3 cells with ZO-2 specific siRNAs (Silencer® select Product # s533910, s18021). Western blot analysis (Fig. a) was performed using whole cell extracts ZO-2 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with ZO-2 Polyclonal Antibody (Product # PA5-17155, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to ZO-2.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ZO-2 in A431 cells using a ZO-2 polyclonal antibody (Product # PA5-17155) (green). Actin filaments are labeled with a fluorescent red phalloidin. DNA is labeled using a fluorescent blue dye.