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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
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- Product number
- PA5-17155 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ZO-2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- It is not recommended to aliquot this antibody.
- Concentration
- 44 µg/mL
Submitted references Curcumin reduces blood-nerve barrier abnormalities and cytotoxicity to endothelial cells and pericytes induced by cisplatin.
Kobutree P, Tothonglor A, Roumwong A, Jindatip D, Agthong S
Folia morphologica 2022 Jul 12;
Folia morphologica 2022 Jul 12;
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on membrane enriched extracts (30 µg lysate) of NIH/3T3 (Lane 1), C2C12 (Lane 2) and tissue extract of Mouse Skeletal Muscle (Lane 3). The blot was probed with Anti-ZO-2 Polyclonal Antibody (Product # PA5-17155, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 160 kDa band corresponding to ZO-2 was observed across the cell lines and tissue tested.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ZO-2 in extracts from C6 and PC-12 cells using ZO-2 polyclonal antibody (Product # PA5-17155).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of ZO-2 was achieved by transfecting NIH/3T3 cells with ZO-2 specific siRNAs (Silencer® select Product # s533910, s18021). Western blot analysis (Fig. a) was performed using whole cell extracts ZO-2 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with ZO-2 Polyclonal Antibody (Product # PA5-17155, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to ZO-2.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ZO-2 in A431 cells using a ZO-2 polyclonal antibody (Product # PA5-17155) (green). Actin filaments are labeled with a fluorescent red phalloidin. DNA is labeled using a fluorescent blue dye.
