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Western blot
Immunocytochemistry
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Immunocytochemistry [1]
- Other assay [1]
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- Product number
- PA1-31072 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SOD2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Rat brain tissue extract was used as a positive control.
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Hamster, Porcine, Rabbit, Xenopus
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 50 µg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Effects of BPA, BPS, and BPF on Oxidative Stress and Antioxidant Enzyme Expression in Bovine Oocytes and Spermatozoa.
Inhibition of NFE2L2-Antioxidant Response Element Pathway by Mitochondrial Reactive Oxygen Species Contributes to Development of Cardiomyopathy and Left Ventricular Dysfunction in Chagas Disease.
Nguyen M, Sabry R, Davis OS, Favetta LA
Genes 2022 Jan 14;13(1)
Genes 2022 Jan 14;13(1)
Inhibition of NFE2L2-Antioxidant Response Element Pathway by Mitochondrial Reactive Oxygen Species Contributes to Development of Cardiomyopathy and Left Ventricular Dysfunction in Chagas Disease.
Wen JJ, Porter C, Garg NJ
Antioxidants & redox signaling 2017 Sep 20;27(9):550-566
Antioxidants & redox signaling 2017 Sep 20;27(9):550-566
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of SOD2 was performed using 70% confluent log phase A549 cells treated with TNF alpha (20 ng/mL for 72h). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with SOD2 Polyclonal Antibody (Product # PA1-31072) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing increased SOD2 expression and localization to the mitochondria. Panel e shows untreated cells with lower expression of SOD2. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 9 Western blot of SOD2 ( A ), GPX1 ( B ), CAT ( C ) protein expression in bovine COCs. For each antioxidant enzyme, a representative blot is seen on the left and densitometric analysis relative to the loading control, beta-actin, is seen on the right. Western blot data represents 4 biological replicates. Different letters indicate significant differences: b denotes statistical significant difference versus a ( p < 0.05). Error bars represent +-SEM.
