GTX630559

antibody from GeneTex

Targeting: SOD2 GClnc1

Immunocytochemistry

Immunohistochemistry

Antibody data

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Validation data

SOD2 protein structure - GTX630559 shown in red.

Enhanced validation

Submitted by: GeneTex (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 15% SDS-PAGE, and the membrane was blotted with SOD2 antibody [GT1433] (GTX630559) diluted at 1:2000.
KD Reagent Type: siRNA
Downregulation: >50%
KD Reagent Prod page: KD Reagent Prod page
KD Reagent Provider: shRNA reagents was provided courtesy of cooperative labs.
KD Reagent Prod no: Proprietary.
Antibody Lot Number: 41589

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: SOD2 antibody [GT1433] detects SOD2 protein by western blot analysis.A. 30 £gg 293T whole cell lysate/extract B. 30 £gg A431 whole cell lysate/extract C. 30 £gg HeLa whole cell lysate/extract |D. 30 £gg HepG2 whole cell lysate/extract12 % SDS-PAGESOD2 antibody [GT1433] (GTX630559) dilution: 1:1000

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: SOD2 antibody [GT1433] detects SOD2 protein by western blot analysis.A. 50 £gg mouse brain lysate/extract12 % SDS-PAGESOD2 antibody [GT1433] (GTX630559) dilution: 1:1000

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: SOD2 antibody [GT1433] detects SOD2 protein by western blot analysis.A. 50 £gg rat brain lysate/extract12 % SDS-PAGESOD2 antibody [GT1433] (GTX630559) dilution: 1:1000

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 15% SDS-PAGE, and the membrane was blotted with SOD2 antibody [GT1433] (GTX630559) diluted at 1:2000.