GTX116093

antibody from GeneTex

Targeting: SOD2 GClnc1

Western blot (Enhanced validation)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX116093

Provider

GeneTex

Proper citation

GeneTex Cat#GTX116093, RRID:AB_10624558

Product name

SOD2 antibody

Antibody type

Polyclonal

Reactivity

Human, Mouse, Rat

Host

Rabbit

References

Submitted references

Changes in Metabolism, Mitochondrial Function, and Oxidative Stress Between Female Rats Under Nonreproductive and 3 Reproductive Conditions.

Hyatt HW, Zhang Y, Hood WR, Kavazis AN
Reproductive sciences (Thousand Oaks, Calif.) 2019 Jan;26(1):114-127

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1,25-Dihydroxyvitamin D₃ modulates the effects of sublethal BPA on mitochondrial function via activating PI3K-Akt pathway and 17β-estradiol secretion in rat granulosa cells.

Lee CT, Wang JY, Chou KY, Hsu MI
The Journal of steroid biochemistry and molecular biology 2019 Jan;185:200-211

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Reduced camptothecin sensitivity of estrogen receptor-positive human breast cancer cells following exposure to di(2-ethylhexyl)phthalate (DEHP) is associated with DNA methylation changes.

Chou CK, Huang HW, Yang CF, Dahms HU, Liang SS, Wang TN, Kuo PL, Hsi E, Tsai EM, Chiu CC
Environmental toxicology 2019 Apr;34(4):401-414

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Upregulation of Znf179 acetylation by SAHA protects cells against oxidative stress.

Wu CC, Lee PT, Kao TJ, Chou SY, Su RY, Lee YC, Yeh SH, Liou JP, Hsu TI, Su TP, Chuang CK, Chang WC, Chuang JY
Redox biology 2018 Oct;19:74-80

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Exogenous C₈-Ceramide Induces Apoptosis by Overproduction of ROS and the Switch of Superoxide Dismutases SOD1 to SOD2 in Human Lung Cancer Cells.

Chang YC, Fong Y, Tsai EM, Chang YG, Chou HL, Wu CY, Teng YN, Liu TC, Yuan SS, Chiu CC
International journal of molecular sciences 2018 Oct 2;19(10)

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Higher incidence of sperm granuloma in the epididymis of C57BL/6N mice.

Park DY, Lee BS, Kim WJ, Im WJ, Han JS, Park H, Yang MJ, Cho JW, Kim DH, Han SC, Kim YB, Son HY
Laboratory animal research 2018 Mar;34(1):20-29

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Laminar shear stress promotes mitochondrial homeostasis in endothelial cells.

Wu LH, Chang HC, Ting PC, Wang DL
Journal of cellular physiology 2018 Jun;233(6):5058-5069

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Dietary Selenomethionine Administration and Its Effects on the American Alligator (Alligator mississippiensis): Oxidative Status and Corticosterone Levels.

Finger JW Jr, Hamilton MT, Kelley MD, Zhang Y, Kavazis AN, Glenn TC, Tuberville TD
Archives of environmental contamination and toxicology 2018 Jul;75(1):37-44

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A mitohormetic response to pro-oxidant exposure in the house mouse.

Zhang Y, Humes F, Almond G, Kavazis AN, Hood WR
American journal of physiology. Regulatory, integrative and comparative physiology 2018 Jan 1;314(1):R122-R134

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Caffeic acid and resveratrol ameliorate cellular damage in cell and Drosophila models of spinocerebellar ataxia type 3 through upregulation of Nrf2 pathway.

Wu YL, Chang JC, Lin WY, Li CC, Hsieh M, Chen HW, Wang TS, Wu WT, Liu CS, Liu KL
Free radical biology & medicine 2018 Feb 1;115:309-317

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High activity before breeding improves reproductive performance by enhancing mitochondrial function and biogenesis.

Zhang Y, Brasher AL, Park NR, Taylor HA, Kavazis AN, Hood WR
The Journal of experimental biology 2018 Apr 6;221(Pt 7)

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Enoyl-CoA hydratase-1 regulates mTOR signaling and apoptosis by sensing nutrients.

Zhang YK, Qu YY, Lin Y, Wu XH, Chen HZ, Wang X, Zhou KQ, Wei Y, Guo F, Yao CF, He XD, Liu LX, Yang C, Guan ZY, Wang SD, Zhao J, Liu DP, Zhao SM, Xu W
Nature communications 2017 Sep 6;8(1):464

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The 1-Week and 8-Month Effects of a Ketogenic Diet or Ketone Salt Supplementation on Multi-Organ Markers of Oxidative Stress and Mitochondrial Function in Rats.

Kephart WC, Mumford PW, Mao X, Romero MA, Hyatt HW, Zhang Y, Mobley CB, Quindry JC, Young KC, Beck DT, Martin JS, McCullough DJ, D'Agostino DP, Lowery RP, Wilson JM, Kavazis AN, Roberts MD
Nutrients 2017 Sep 15;9(9)

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ERRα Maintains Mitochondrial Oxidative Metabolism and Constitutes an Actionable Target in PGC1α-Elevated Melanomas.

Luo C, Balsa E, Thomas A, Hatting M, Jedrychowski M, Gygi SP, Widlund HR, Puigserver P
Molecular cancer research : MCR 2017 Oct;15(10):1366-1375

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Specificity protein 1-modulated superoxide dismutase 2 enhances temozolomide resistance in glioblastoma, which is independent of O⁶-methylguanine-DNA methyltransferase.

Chang KY, Hsu TI, Hsu CC, Tsai SY, Liu JJ, Chou SW, Liu MS, Liou JP, Ko CY, Chen KY, Hung JJ, Chang WC, Chuang CK, Kao TJ, Chuang JY
Redox biology 2017 Oct;13:655-664

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No Effect of Lifelong Methylmercury Exposure on Oxidative Status in Zebra Finches (Taeniopygia guttata): A Demonstration of Methylmercury-Induced Selection?

Finger JW Jr, Botero J, Zhang Y, Still SE, Hoffman AJ, Kavazis AN, Cristol DA, Wada H
Bulletin of environmental contamination and toxicology 2017 Dec;99(6):668-672

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Lactation has persistent effects on a mother's metabolism and mitochondrial function.

Hyatt HW, Zhang Y, Hood WR, Kavazis AN
Scientific reports 2017 Dec 7;7(1):17118

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Comparative changes in antioxidant enzymes and oxidative stress in cardiac, fast twitch and slow twitch skeletal muscles following endurance exercise training.

Hyatt HW, Smuder AJ, Sollanek KJ, Morton AB, Roberts MD, Kavazis AN
International journal of physiology, pathophysiology and pharmacology 2016;8(4):160-168

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A Ketogenic Diet in Rodents Elicits Improved Mitochondrial Adaptations in Response to Resistance Exercise Training Compared to an Isocaloric Western Diet.

Hyatt HW, Kephart WC, Holland AM, Mumford P, Mobley CB, Lowery RP, Roberts MD, Wilson JM, Kavazis AN
Frontiers in physiology 2016;7:533

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Reproduction Does Not Adversely Affect Liver Mitochondrial Respiratory Function but Results in Lipid Peroxidation and Increased Antioxidants in House Mice.

Mowry AV, Kavazis AN, Sirman AE, Potts WK, Hood WR
PloS one 2016;11(8):e0160883

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Influence of endurance exercise training on antioxidant enzymes, tight junction proteins, and inflammatory markers in the rat ileum.

Holland AM, Hyatt HW, Smuder AJ, Sollanek KJ, Morton AB, Roberts MD, Kavazis AN
BMC research notes 2015 Sep 30;8:514

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Comparative adaptations in oxidative and glycolytic muscle fibers in a low voluntary wheel running rat model performing three levels of physical activity.

Hyatt HW, Toedebusch RG, Ruegsegger G, Mobley CB, Fox CD, McGinnis GR, Quindry JC, Booth FW, Roberts MD, Kavazis AN
Physiological reports 2015 Nov;3(11)

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Quantitative Proteomics of Human Fibroblasts with I1061T Mutation in Niemann-Pick C1 (NPC1) Protein Provides Insights into the Disease Pathogenesis.

Rauniyar N, Subramanian K, Lavallée-Adam M, Martínez-Bartolomé S, Balch WE, Yates JR 3rd
Molecular & cellular proteomics : MCP 2015 Jul;14(7):1734-49

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Sequential actions of SIRT1-RELB-SIRT3 coordinate nuclear-mitochondrial communication during immunometabolic adaptation to acute inflammation and sepsis.

Liu TF, Vachharajani V, Millet P, Bharadwaj MS, Molina AJ, McCall CE
The Journal of biological chemistry 2015 Jan 2;290(1):396-408

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Increased antioxidant defense mechanism in human adventitia-derived progenitor cells is associated with therapeutic benefit in ischemia.

Iacobazzi D, Mangialardi G, Gubernator M, Hofner M, Wielscher M, Vierlinger K, Reni C, Oikawa A, Spinetti G, Vono R, Sangalli E, Montagnani M, Madeddu P
Antioxidants & redox signaling 2014 Oct 10;21(11):1591-604

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SIRT1 activating compounds reduce oxidative stress mediated neuronal loss in viral induced CNS demyelinating disease.

Khan RS, Dine K, Das Sarma J, Shindler KS
Acta neuropathologica communications 2014 Jan 2;2:3

Western blot

Enhanced validation

Submitted by

GeneTex (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 15% SDS-PAGE, and the membrane was blotted with SOD2 antibody (GTX116093) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

KD Reagent Type

siRNA

Downregulation

>50%

KD Reagent Provider

shRNA reagents was provided courtesy of cooperative labs.

KD Reagent Prod page

KD Reagent Prod page

KD Reagent Prod no

Proprietary.

Antibody Lot Number

41801

Show more

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE GTX116093 diluted at 1:1000

Validation comment

WB

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (50 ug of whole cell lysate)    A: Rat brain   12% SDS PAGE    GTX116093 diluted at 1:1000    

Validation comment

WB

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (30 ug of whole cell lysate) A: Hela B: HCT116 15% SDS PAGE GTX116093 diluted at 1:1000

Validation comment

WB

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

SOD2 antibody detects SOD2 protein by western blot analysis. Various whole cell extracts (30 ?g) were separated by 15% SDS-PAGE, and the membrane was blotted with SOD2 antibody (GTX116093) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

SOD2 antibody detects SOD2 protein by western blot analysis. Mouse tissue extracts (50 ?g) was separated by 15% SDS-PAGE, and the membrane was blotted with SOD2 antibody (GTX116093) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

SOD2 antibody detects SOD2 protein by western blot analysis. Rat tissue extracts (50 ?g) was separated by 15% SDS-PAGE, and the membrane was blotted with SOD2 antibody (GTX116093) diluted by 1:2500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 15% SDS-PAGE, and the membrane was blotted with SOD2 antibody (GTX116093) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunofluorescence analysis of methanol-fixed HeLa, using SOD2/ Mn SOD(GTX116093) antibody at 1:200 dilution.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

SOD2 antibody detects SOD2 protein at mitochondria in human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. SOD2 antibody (GTX116093) diluted at 1:500.