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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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- Product number
- GTX31147 - Provider product page
- Provider
- GeneTex
- Product name
- SOD2 antibody [1H6]
- Antibody type
- Monoclonal
- Reactivity
- Human
- Host
- Mouse
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Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- WB analysis of various cell lysates (50ug per lane) using SOD2 antibody [1H6] at a dilution of 1:500.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ICC/IF analysis of HUVEC cells with (right) or without (left) SOD2 antibody [1H6] at a dilution of 1:200 (green). F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with DAPI.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ICC/IF analysis of HeLa cells with (right) or without (left) SOD2 antibody [1H6] at a dilution of 1:200 (green). F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with DAPI.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- IP analysis was performed with 500£gg of HepG2 lysate and 2 £gg of SOD2 antibody [1H6]. HepG2 lysate and the precipitate were detected by the same antibody with EasyBlot as secondary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- IHC-P analysis of human cervical carcinoma with (right) or without (left) SOD2 antibody [1H6] at a dilution of 1:200. To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- IHC-P analysis of human liver tissue qith (right) or without (left) SOD2 antibody [1H6] at a dilution of 1:200. To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min.
