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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- F54485 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Superoxide Dismutase 2 Antibody / SOD2
- Antibody type
- Monoclonal
- Description
- Protein G affinity
- Reactivity
- Human, Mouse
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 0.05 µl, 0.2 µl
- Storage
- Aliquot the Superoxide Dismutase 2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human brain, 2) human HeLa, 3) human MCF7, 4) mouse NIH 3T3 and 5) human SH-SY5Y lysate with Superoxide Dismutase 2 antibody. Predicted molecular weight ~25 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human brain tissue with Superoxide Dismutase 2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human skeletal muscle tissue with Superoxide Dismutase 2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of fixed and permeabilized human A549 cells with Superoxide Dismutase 2 antibody; Blue=isotype control, Green= Superoxide Dismutase 2 antibody.
