F54485

antibody from NSJ Bioreagents

Targeting: SOD2 GClnc1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

F54485

Provider

NSJ Bioreagents

Product name

Superoxide Dismutase 2 Antibody / SOD2 / SODM

Antibody type

Monoclonal

Description

This highly specific Superoxide Dismutase 2 antibody is suitable for use in Western blot/Immunohistochemistry/Flow cytometry applications with human and mouse samples.

Reactivity

Human, Mouse

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Antibody clone number

1559CT572.3.83

Vial size

0.05 ml, 0.2 ml

Concentration

In 1X PBS, pH 7.4, with 0.09% sodium azide

Storage

Aliquot the Superoxide Dismutase 2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) human brain, 2) human HeLa, 3) human MCF7, 4) mouse NIH 3T3 and 5) human SH-SY5Y lysate with Superoxide Dismutase 2 antibody. Predicted molecular weight ~25 kDa.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human brain tissue with Superoxide Dismutase 2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human skeletal muscle tissue with Superoxide Dismutase 2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of fixed and permeabilized human A549 cells with Superoxide Dismutase 2 antibody; Blue=isotype control, Green= Superoxide Dismutase 2 antibody.