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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
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- Product number
- Ab169025 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- SOD2 Mouse mAb
- Antibody type
- Monoclonal
- Description
- ApplicationWB: 1/1,000 - 1/2,000; Predicted molecular weight: 24.8 KDaICC/IF: 1/200
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- I01/6C1
- Vial size
- 100_l,10_l,1ml,50_l
- Concentration
- 1 mg/ml
- Storage
- Store at 4¡C short term (1-2 weeks). Store at -20¡C or -80¡C. Avoid freeze / thaw cycle .
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Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- SOD2 Mouse mAb (Ab169025) - Western Blot All lanes: SOD2 Mouse Antibody (Ab169025) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution Predicted band size: 25 kDa Observed band size: 23 kDa Exposure time: 38.2 s
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- SOD2 Mouse mAb (Ab169025) - Western Blot All lanes: SOD2 Mouse Antibody (Ab169025) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution Predicted band size: 25 kDa Observed band size: 23 kDa Exposure time: 38.2 s
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- SOD2 Mouse mAb (Ab169025) - ICC/IF IF analysis of SOD2 (Green) in U-2 OS cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with SOD2 Mouse mAb (Ab169025) at 5.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/500 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.
