- PA5-30604 - Invitrogen Antibodies SOD2 antibody

Submitted references Preventive Effect of Limosilactobacillus fermentum SCHY34 on Lead Acetate-Induced Neurological Damage in SD Rats.

Long X, Wu H, Zhou Y, Wan Y, Kan X, Gong J, Zhao X
Frontiers in nutrition 2022;9:852012

Pre-treatment With PLGA/Silibinin Nanoparticles Mitigates Dacarbazine-Induced Hepatotoxicity.

Durymanov M, Permyakova A, Reineke J
Frontiers in bioengineering and biotechnology 2020;8:495

Oxidative stress and mitochondrial uncoupling protein 2 expression in hepatic steatosis induced by exposure to xenobiotic DDE and high fat diet in male Wistar rats.

Migliaccio V, Scudiero R, Sica R, Lionetti L, Putti R
PloS one 2019;14(4):e0215955

Extracts of Physalis peruviana Protect Astrocytic Cells Under Oxidative Stress With Rotenone.

Areiza-Mazo N, Robles J, Zamudio-Rodriguez JA, Giraldez L, Echeverria V, Barrera-Bailon B, Aliev G, Sahebkar A, Ashraf GM, Barreto GE
Frontiers in chemistry 2018;6:276

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of SOD2 using 30 µg of A) HeLa and B) HCT116 lysate. Samples were loaded onto a 15% SDS-PAGE gel and probed with a SOD2 polyclonal antibody (Product # PA5-30604) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of SOD2 using 50 µg of mouse brain lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a SOD2 polyclonal antibody (Product # PA5-30604) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of SOD2 using 50 µg of rat brain lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a SOD2 polyclonal antibody (Product # PA5-30604) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of SOD2 was performed by separating 30 µg of various whole cell extracts by 15% SDS-PAGE. Proteins were transferred to a membrane and probed with a SOD2 Polyclonal Antibody (Product # PA5-30604) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of SOD2 was performed by separating 50 µg of mouse tissue extracts by 15% SDS-PAGE. Proteins were transferred to a membrane and probed with a SOD2 Polyclonal Antibody (Product # PA5-30604) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of SOD2 was performed by separating 50 µg of rat tissue extracts by 15% SDS-PAGE. Proteins were transferred to a membrane and probed with a SOD2 Polyclonal Antibody (Product # PA5-30604) at a dilution of 1:2500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of SOD2 was performed by separating 30 µg of various whole cell extracts by 15% SDS-PAGE. Proteins were transferred to a membrane and probed with a SOD2 Polyclonal Antibody (Product # PA5-30604) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis was performed on whole cell extracts of A549 (Lane 1), A549 treated with TNFa (20 ng/mL, 72hrs) (Lane 2), HEK 293 (Lane 3), MCF-7 (Lane 4) and T-47D (Lane 5). The blot was probed with SOD2 Polyclonal Antibody (Product # PA5-30604, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 21 kDa band corresponding to SOD2 was observed in all the lanes, with A549 showing an increase in expression upon TNFa treatment.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of SOD2 in methanol-fixed HeLa cells using a SOD2 polyclonal antibody (Product # PA5-30604) at a 1:200 dilution.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry (Paraffin) analysis of SOD2 was performed in paraffin-embedded human breast carcinoma tissue using SOD2 Polyclonal Antibody (Product # PA5-30604) at a dilution of 1:500.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 4 Expression of antioxidant defense and ERK proteins in T98G cells. (A) Expression of Superoxide dismutase (SOD) and Catalase determined by Western Blot in T98G cell cultures after co-treatment with 50 muM rotenone plus EF and AD at 25 mu g/ml. (B) Expression of Glutathione determined by Western Blot in T98G cell cultures after co-treatment with 50 muM rotenone plus EF and AD at 25 mu g/ml. (C) Expression of ERK and MAPK proteins determined by Western blotting on T98G cells after co-treatment with rotenone plus EF and AD at 25 mu g/ml.

PA5-30604

Antibody data