Explore
Validate
Learn
Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 14-9788-80 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-Cytokeratin 6 Monoclonal Antibody (LL020), eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The monoclonal antibody LL020 recognizes human Cytokeratin 6. Cytokeratins are intermediate filament proteins comprising one component of the cytoskeleton. Cytokeratin 6 is a type II (basic) keratin that associates with Cytokeratin 16 and Cytokeratin 17. Cytokeratin 6 is expressed in the epidermis, outer root sheath of hair follicles, oral mucosa, suprabasal cells of stratified epithelia, and glandular epithelia. Gene mutations result in inherited skin and hair disorders. Some reports suggest that LL020 may have some crossreactivity to Cytokeratin 5. Applications Reported: This LL020 antibody has been reported for use in western blotting, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, microscopy, immunohistochemical staining, and ELISA. Applications Tested: This LL020 antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded human tissue using low pH antigen retrieval and can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgM
- Antibody clone number
- LL020
- Vial size
- 25 µg
- Concentration
- 0.5 mg/mL
- Storage
- 4° C
Submitted references Hedgehog signaling, keratin 6 induction, and sebaceous gland morphogenesis: implications for pachyonychia congenita and related conditions.
Interleukin-1 induces transcription of keratin K6 in human epidermal keratinocytes.
Keratins and the skin.
Keratins (K16 and K17) as markers of keratinocyte hyperproliferation in psoriasis in vivo and in vitro.
Gu LH, Coulombe PA
The American journal of pathology 2008 Sep;173(3):752-61
The American journal of pathology 2008 Sep;173(3):752-61
Interleukin-1 induces transcription of keratin K6 in human epidermal keratinocytes.
Komine M, Rao LS, Freedberg IM, Simon M, Milisavljevic V, Blumenberg M
The Journal of investigative dermatology 2001 Feb;116(2):330-8
The Journal of investigative dermatology 2001 Feb;116(2):330-8
Keratins and the skin.
Fuchs E
Annual review of cell and developmental biology 1995;11:123-53
Annual review of cell and developmental biology 1995;11:123-53
Keratins (K16 and K17) as markers of keratinocyte hyperproliferation in psoriasis in vivo and in vitro.
Leigh IM, Navsaria H, Purkis PE, McKay IA, Bowden PE, Riddle PN
The British journal of dermatology 1995 Oct;133(4):501-11
The British journal of dermatology 1995 Oct;133(4):501-11
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of formalin-fixed paraffin embedded human skin using 10 µg/mL Mouse IgM Isotype Control Purified (left) or 10 µg/mL of Anti-Human Cytokeratin 6 Purified (right) followed by Anti-Mouse Biotin, Streptavidin-HRP, and DAB visualization.Nuclei are counterstained with hematoxylin.
