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- Antibody Data
- Antigen structure
- References [23]
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- Western blot [9]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- GTX100554 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX100554, RRID:AB_10618670
- Product name
- SOD1 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references Changes in Metabolism, Mitochondrial Function, and Oxidative Stress Between Female Rats Under Nonreproductive and 3 Reproductive Conditions.
Liraglutide protects against glucolipotoxicity-induced RIN-m5F β-cell apoptosis through restoration of PDX1 expression.
Upregulation of Znf179 acetylation by SAHA protects cells against oxidative stress.
Hepatic ZIP8 deficiency is associated with disrupted selenium homeostasis, liver pathology, and tumor formation.
Dietary Selenomethionine Administration and Its Effects on the American Alligator (Alligator mississippiensis): Oxidative Status and Corticosterone Levels.
SNX10 mediates alcohol-induced liver injury and steatosis by regulating the activation of chaperone-mediated autophagy.
Aβ exacerbates α-synuclein-induced neurotoxicity through impaired insulin signaling in α-synuclein-overexpressed human SK-N-MC neuronal cells.
A mitohormetic response to pro-oxidant exposure in the house mouse.
Caffeic acid and resveratrol ameliorate cellular damage in cell and Drosophila models of spinocerebellar ataxia type 3 through upregulation of Nrf2 pathway.
Reduced expression of SIRT1 and SOD-1 and the correlation between these levels in various regions of the brains of patients with Alzheimer's disease.
High activity before breeding improves reproductive performance by enhancing mitochondrial function and biogenesis.
3, 4-dihydroxybenzalacetone attenuates lipopolysaccharide-induced inflammation in acute lung injury via down-regulation of MMP-2 and MMP-9 activities through suppressing ROS-mediated MAPK and PI3K/AKT signaling pathways.
3,4-Dihydroxybenzalactone Suppresses Human Non-Small Cell Lung Carcinoma Cells Metastasis via Suppression of Epithelial to Mesenchymal Transition, ROS-Mediated PI3K/AKT/MAPK/MMP and NFκB Signaling Pathways.
Zinc regulates Nox1 expression through a NF-κB and mitochondrial ROS dependent mechanism to induce senescence of vascular smooth muscle cells.
No Effect of Lifelong Methylmercury Exposure on Oxidative Status in Zebra Finches (Taeniopygia guttata): A Demonstration of Methylmercury-Induced Selection?
Mitochondrial degeneration precedes the development of muscle atrophy in progression of cancer cachexia in tumour-bearing mice.
Lactation has persistent effects on a mother's metabolism and mitochondrial function.
Comparative changes in antioxidant enzymes and oxidative stress in cardiac, fast twitch and slow twitch skeletal muscles following endurance exercise training.
Reproduction Does Not Adversely Affect Liver Mitochondrial Respiratory Function but Results in Lipid Peroxidation and Increased Antioxidants in House Mice.
Blackberry, raspberry and black raspberry polyphenol extracts attenuate angiotensin II-induced senescence in vascular smooth muscle cells.
Selenite and ebselen supplementation attenuates D-galactose-induced oxidative stress and increases expression of SELR and SEP15 in rat lens.
Oxidative DNA damage in kidneys and heart of hypertensive mice is prevented by blocking angiotensin II and aldosterone receptors.
Aldosterone activates transcription factor Nrf2 in kidney cells both in vitro and in vivo.
Hyatt HW, Zhang Y, Hood WR, Kavazis AN
Reproductive sciences (Thousand Oaks, Calif.) 2019 Jan;26(1):114-127
Reproductive sciences (Thousand Oaks, Calif.) 2019 Jan;26(1):114-127
Liraglutide protects against glucolipotoxicity-induced RIN-m5F β-cell apoptosis through restoration of PDX1 expression.
Kornelius E, Li HH, Peng CH, Yang YS, Chen WJ, Chang YZ, Bai YC, Liu S, Huang CN, Lin CL
Journal of cellular and molecular medicine 2019 Jan;23(1):619-629
Journal of cellular and molecular medicine 2019 Jan;23(1):619-629
Upregulation of Znf179 acetylation by SAHA protects cells against oxidative stress.
Wu CC, Lee PT, Kao TJ, Chou SY, Su RY, Lee YC, Yeh SH, Liou JP, Hsu TI, Su TP, Chuang CK, Chang WC, Chuang JY
Redox biology 2018 Oct;19:74-80
Redox biology 2018 Oct;19:74-80
Hepatic ZIP8 deficiency is associated with disrupted selenium homeostasis, liver pathology, and tumor formation.
Liu L, Geng X, Cai Y, Copple B, Yoshinaga M, Shen J, Nebert DW, Wang H, Liu Z
American journal of physiology. Gastrointestinal and liver physiology 2018 Oct 1;315(4):G569-G579
American journal of physiology. Gastrointestinal and liver physiology 2018 Oct 1;315(4):G569-G579
Dietary Selenomethionine Administration and Its Effects on the American Alligator (Alligator mississippiensis): Oxidative Status and Corticosterone Levels.
Finger JW Jr, Hamilton MT, Kelley MD, Zhang Y, Kavazis AN, Glenn TC, Tuberville TD
Archives of environmental contamination and toxicology 2018 Jul;75(1):37-44
Archives of environmental contamination and toxicology 2018 Jul;75(1):37-44
SNX10 mediates alcohol-induced liver injury and steatosis by regulating the activation of chaperone-mediated autophagy.
You Y, Li WZ, Zhang S, Hu B, Li YX, Li HD, Tang HH, Li QW, Guan YY, Liu LX, Bao WL, Shen X
Journal of hepatology 2018 Jul;69(1):129-141
Journal of hepatology 2018 Jul;69(1):129-141
Aβ exacerbates α-synuclein-induced neurotoxicity through impaired insulin signaling in α-synuclein-overexpressed human SK-N-MC neuronal cells.
Chang CC, Li HH, Chang YT, Ho YJ, Hsieh LJ, Chiu PY, Cheng YS, Lin CL, Lai TJ
CNS neuroscience & therapeutics 2018 Jan;24(1):47-57
CNS neuroscience & therapeutics 2018 Jan;24(1):47-57
A mitohormetic response to pro-oxidant exposure in the house mouse.
Zhang Y, Humes F, Almond G, Kavazis AN, Hood WR
American journal of physiology. Regulatory, integrative and comparative physiology 2018 Jan 1;314(1):R122-R134
American journal of physiology. Regulatory, integrative and comparative physiology 2018 Jan 1;314(1):R122-R134
Caffeic acid and resveratrol ameliorate cellular damage in cell and Drosophila models of spinocerebellar ataxia type 3 through upregulation of Nrf2 pathway.
Wu YL, Chang JC, Lin WY, Li CC, Hsieh M, Chen HW, Wang TS, Wu WT, Liu CS, Liu KL
Free radical biology & medicine 2018 Feb 1;115:309-317
Free radical biology & medicine 2018 Feb 1;115:309-317
Reduced expression of SIRT1 and SOD-1 and the correlation between these levels in various regions of the brains of patients with Alzheimer's disease.
Cao K, Dong YT, Xiang J, Xu Y, Hong W, Song H, Guan ZZ
Journal of clinical pathology 2018 Dec;71(12):1090-1099
Journal of clinical pathology 2018 Dec;71(12):1090-1099
High activity before breeding improves reproductive performance by enhancing mitochondrial function and biogenesis.
Zhang Y, Brasher AL, Park NR, Taylor HA, Kavazis AN, Hood WR
The Journal of experimental biology 2018 Apr 6;221(Pt 7)
The Journal of experimental biology 2018 Apr 6;221(Pt 7)
3, 4-dihydroxybenzalacetone attenuates lipopolysaccharide-induced inflammation in acute lung injury via down-regulation of MMP-2 and MMP-9 activities through suppressing ROS-mediated MAPK and PI3K/AKT signaling pathways.
Chao W, Deng JS, Huang SS, Li PY, Liang YC, Huang GJ
International immunopharmacology 2017 Sep;50:77-86
International immunopharmacology 2017 Sep;50:77-86
3,4-Dihydroxybenzalactone Suppresses Human Non-Small Cell Lung Carcinoma Cells Metastasis via Suppression of Epithelial to Mesenchymal Transition, ROS-Mediated PI3K/AKT/MAPK/MMP and NFκB Signaling Pathways.
Chao W, Deng JS, Li PY, Liang YC, Huang GJ
Molecules (Basel, Switzerland) 2017 Mar 28;22(4)
Molecules (Basel, Switzerland) 2017 Mar 28;22(4)
Zinc regulates Nox1 expression through a NF-κB and mitochondrial ROS dependent mechanism to induce senescence of vascular smooth muscle cells.
Salazar G, Huang J, Feresin RG, Zhao Y, Griendling KK
Free radical biology & medicine 2017 Jul;108:225-235
Free radical biology & medicine 2017 Jul;108:225-235
No Effect of Lifelong Methylmercury Exposure on Oxidative Status in Zebra Finches (Taeniopygia guttata): A Demonstration of Methylmercury-Induced Selection?
Finger JW Jr, Botero J, Zhang Y, Still SE, Hoffman AJ, Kavazis AN, Cristol DA, Wada H
Bulletin of environmental contamination and toxicology 2017 Dec;99(6):668-672
Bulletin of environmental contamination and toxicology 2017 Dec;99(6):668-672
Mitochondrial degeneration precedes the development of muscle atrophy in progression of cancer cachexia in tumour-bearing mice.
Brown JL, Rosa-Caldwell ME, Lee DE, Blackwell TA, Brown LA, Perry RA, Haynie WS, Hardee JP, Carson JA, Wiggs MP, Washington TA, Greene NP
Journal of cachexia, sarcopenia and muscle 2017 Dec;8(6):926-938
Journal of cachexia, sarcopenia and muscle 2017 Dec;8(6):926-938
Lactation has persistent effects on a mother's metabolism and mitochondrial function.
Hyatt HW, Zhang Y, Hood WR, Kavazis AN
Scientific reports 2017 Dec 7;7(1):17118
Scientific reports 2017 Dec 7;7(1):17118
Comparative changes in antioxidant enzymes and oxidative stress in cardiac, fast twitch and slow twitch skeletal muscles following endurance exercise training.
Hyatt HW, Smuder AJ, Sollanek KJ, Morton AB, Roberts MD, Kavazis AN
International journal of physiology, pathophysiology and pharmacology 2016;8(4):160-168
International journal of physiology, pathophysiology and pharmacology 2016;8(4):160-168
Reproduction Does Not Adversely Affect Liver Mitochondrial Respiratory Function but Results in Lipid Peroxidation and Increased Antioxidants in House Mice.
Mowry AV, Kavazis AN, Sirman AE, Potts WK, Hood WR
PloS one 2016;11(8):e0160883
PloS one 2016;11(8):e0160883
Blackberry, raspberry and black raspberry polyphenol extracts attenuate angiotensin II-induced senescence in vascular smooth muscle cells.
Feresin RG, Huang J, Klarich DS, Zhao Y, Pourafshar S, Arjmandi BH, Salazar G
Food & function 2016 Oct 12;7(10):4175-4187
Food & function 2016 Oct 12;7(10):4175-4187
Selenite and ebselen supplementation attenuates D-galactose-induced oxidative stress and increases expression of SELR and SEP15 in rat lens.
Dai J, Zhou J, Liu H, Huang K
Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry 2016 Dec;21(8):1037-1046
Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry 2016 Dec;21(8):1037-1046
Oxidative DNA damage in kidneys and heart of hypertensive mice is prevented by blocking angiotensin II and aldosterone receptors.
Brand S, Amann K, Mandel P, Zimnol A, Schupp N
PloS one 2014;9(12):e115715
PloS one 2014;9(12):e115715
Aldosterone activates transcription factor Nrf2 in kidney cells both in vitro and in vivo.
Queisser N, Oteiza PI, Link S, Hey V, Stopper H, Schupp N
Antioxidants & redox signaling 2014 Nov 20;21(15):2126-42
Antioxidants & redox signaling 2014 Nov 20;21(15):2126-42
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Enhanced validation
Supportive validation
- Submitted by
- GeneTex (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 15% SDS-PAGE, and the membrane was blotted with SOD1 antibody (GTX100554) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (50 ?g of whole cell lysate) A: mouse brain 15% SDS PAGE GTX100554 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A: HeLa 15% SDS PAGE Superoxide Dismutase 1 antibody GTX100554 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (50 ?g of whole cell lysate) A: Rat brain 15% SDS PAGE GTX100554 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis of SOD1 (GTX100554, upper panel) and beta-actin (GTX109639, lower panel)? Sample (10 ?g of whole cell lysate)? A: HeLa mock control? B: HeLa transfected shSOD1 15% SDS PAGE? GTX100554 diluted at 1:500 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- SOD1 antibody detects SOD1 protein by western blot analysis. Whole cell extracts (30 ?g) was separated by 15% SDS-PAGE, and the membrane was blotted with SOD1 antibody (GTX100554) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- SOD1 antibody detects SOD1 protein by western blot analysis. Various whole cell extracts (30 ?g) were separated by 15% SDS-PAGE, and the membrane was blotted with SOD1 antibody (GTX100554) diluted at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 15% SDS-PAGE, and the membrane was blotted with SOD1 antibody (GTX100554) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis of SOD1 (GTX100554, upper panel) and beta-actin (GTX109639, lower panel)? Sample (10 ?g of whole cell lysate)? A: HeLa mock control? B: HeLa transfected shSOD1 15% SDS PAGE? GTX100554 diluted at 1:500 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ELISA detection of SOD1 using GTX100554 for capture at a concentration of 5 ?g/mL and GTX89049 for detection at a concentration of 1.5 ?g/mL.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- SOD1 antibody detects SOD1 protein at nucleus by immunofluorescent analysis.Sample: U-87 MG cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: SOD1 protein stained by SOD1 antibody (GTX100554) diluted at 1:500.Red: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody (GTX631836) diluted at 1:200.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- SOD1 antibody detects SOD1 protein at cytosol on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. SOD1 antibody (GTX100554) dilution: 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence photomicrographs of paraffin-embedded mouse fetal brain.Green: SOD1 antibody (GTX100554) diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488) (GTX213110-04).Red: beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:100. The signal was developed using goat anti-mouse IgG antibody (Dylight594) (GTX213111-05).Blue: Nuclear staining with Hoechst 33342.
