Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- AF3787 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse/Rat SOD1/Cu-Zn SOD Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects endogenous mouse and rat SOD1 in Western blots. In Western blots, this antibody shows no cross-reacivity with recombinant human SOD2 or SOD3.
- Reactivity
- Mouse, Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P08228
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references MAP4K4 Activation Mediates Motor Neuron Degeneration in Amyotrophic Lateral Sclerosis.
Paeoniflorin Attenuated Oxidative Stress in Rat COPD Model Induced by Cigarette Smoke.
Adverse effects of reduced oxygen tension on the proliferative capacity of rat kidney and insulin-secreting cell lines involve DNA damage and stress responses.
Maternal diet influences DNA damage, aortic telomere length, oxidative stress, and antioxidant defense capacity in rats.
Wu C, Watts ME, Rubin LL
Cell reports 2019 Jan 29;26(5):1143-1156.e5
Cell reports 2019 Jan 29;26(5):1143-1156.e5
Paeoniflorin Attenuated Oxidative Stress in Rat COPD Model Induced by Cigarette Smoke.
Lin J, Xu F, Wang G, Kong L, Luo Q, Lv Y, Liu J, Wei Y, Li L, Zhang H, Dong J
Evidence-based complementary and alternative medicine : eCAM 2016;2016:1698379
Evidence-based complementary and alternative medicine : eCAM 2016;2016:1698379
Adverse effects of reduced oxygen tension on the proliferative capacity of rat kidney and insulin-secreting cell lines involve DNA damage and stress responses.
Chen JH, Jones RH, Tarry-Adkins J, Smith NH, Ozanne SE
Experimental cell research 2008 Oct 1;314(16):3075-80
Experimental cell research 2008 Oct 1;314(16):3075-80
Maternal diet influences DNA damage, aortic telomere length, oxidative stress, and antioxidant defense capacity in rats.
Tarry-Adkins JL, Martin-Gronert MS, Chen JH, Cripps RL, Ozanne SE
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2008 Jun;22(6):2037-44
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2008 Jun;22(6):2037-44
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Supportive validation
- Submitted by
- R&D Systems (provider)
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- Experimental details
- Detection of Mouse/Rat SOD1/Cu-Zn SOD by Western Blot. Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line and NRK rat normal kidney cell line. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse/Rat SOD1/Cu-Zn SOD Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3787) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for SOD1/Cu-Zn SOD at approximately 19 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2.