PAB29364

antibody from Abnova Corporation

Targeting: SOD1 ALS, ALS1, IPOA

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB29364

Provider

Abnova Corporation

Product name

SOD1 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant human SOD1.

Antigen sequence

PVQGIINFEQKESNGPVKVWGSIKGLTEGLHGFHV
HEFGDNTAGCTSAGPHFNPLSRKHGGPKDEERHVG
DLGNVTADKDGVADVSIEDSVISLSGDHCIIGRTL
VVHEKADDLGKGGNEESTKTGNAGSRLACG


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells) Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells) Lane 3: PC12 cell lysate (Pheochromocytoma of rat adrenal medulla) with SOD1 polyclonal antibody (Cat # PAB29364) at 1:250-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-251 MG with SOD1 polyclonal antibody (Cat # PAB29364) at 1-4 ug/mL concentration shows positivity in cytoplasm and nucleus but excluded from the nucleoli.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human liver with SOD1 polyclonal antibody (Cat # PAB29364) shows nuclear and cytoplasmic positivity in hepatocytes at 1:2500-1:5000 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)