Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Flow cytometry [1]
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Validation data
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- Product number
- MAB10394 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB10394, RRID:AB_11183146
- Product name
- SOD1 monoclonal antibody, clone 6F5
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against recombinant SOD1.
- Isotype
- IgG
- Antibody clone number
- 6F5
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of SOD1 monoclonal antobody, clone 6F5 (Cat # MAB10394) against HeLa (1), NIH/3T3 (2), A-549 (3) and A-431 (4) cell lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Confocal immunofluorescence analysis of 3T3-L1 cells using SOD1 monoclonal antobody, clone 6F5 (Cat # MAB10394) (green). Blue: DRAQ5 fluorescent DNA dye.
- Validation comment
- Immunofluorescence
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Confocal immunofluorescence analysis of PANC-1 (A) and SK-BR-3 (B) cells using SOD1 monoclonal antobody, clone 6F5 (Cat # MAB10394) (green).Red: Actin filaments have been labeled with DY-554 phalloidin.Blue: DRAQ5 fluorescent DNA dye.
- Validation comment
- Immunofluorescence
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Flow cytometric analysis of A-431 cells using SOD1 monoclonal antobody, clone 6F5 (Cat # MAB10394) (green) and negative control (purple).
- Validation comment
- Flow Cytometry