Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [13]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Other assay [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-85095 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SOD1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Antigen Retrieval (IF): citrate buffer, pH 6.0, 15 min, and IHC: EDTA based, pH 8.0 buffer, 15min. Keep as concentrated solution. Predicted reactivity: Mouse (81%), Rat (81%), Pig (80%), Rabbit (82%), Rhesus Monkey (91%), Chimpanzee (100%). Positive Control: 293T, HeLa, NT2D1, SK-N-AS, IMR32, SK-N-SH, U87-MG, mouse brain, Rat brain, F79, F123. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat, Chicken/Avian
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.15 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references β-Nicotinamide Mononucleotide (NMN) Administrated by Intraperitoneal Injection Mediates Protection Against UVB-Induced Skin Damage in Mice.
Zhou X, Du HH, Long X, Pan Y, Hu J, Yu J, Zhao X
Journal of inflammation research 2021;14:5165-5182
Journal of inflammation research 2021;14:5165-5182
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SOD1 in A) HeLa cells using SOD1 polyclonal antibody (Product # PA5-85095) using 30 µg of sample at a dilution of 1:1000. Sample was then incubated with HRP-conjugated anti-rabbit IgG secondary antibody. Prior to incubation with primary antibody, the sample was separated on 15% SDS-PAGE.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SOD1 in various whole cell extracts using SOD1 polyclonal antibody (Product # PA5-85095) using 30 µg of sample at a dilution of 1:1000. Sample was then incubated with HRP-conjugated anti-rabbit IgG secondary antibody. Prior to incubation with primary antibody, the sample was separated on 15% SDS-PAGE.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SOD1 in non-transfected (–) and transfected (+) 293T whole cell extracts using SOD1 polyclonal antibody (Product # PA5-85095) using 30 µg of sample at a dilution of 1:5000. Sample was then incubated with HRP-conjugated anti-rabbit IgG secondary antibody. Prior to incubation with primary antibody, the sample was separated on 15% SDS-PAGE.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SOD1 in A) mouse brain using SOD1 polyclonal antibody (Product # PA5-85095) using 50 µg of sample at a dilution of 1:1000. Sample was then incubated with HRP-conjugated anti-rabbit IgG secondary antibody. Prior to incubation with primary antibody, the sample was separated on 15% SDS-PAGE.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SOD1 in whole cell extracts using SOD1 polyclonal antibody (Product # PA5-85095) using 30 µg of sample at a dilution of 1:1000. Sample was then incubated with HRP-conjugated anti-rabbit IgG secondary antibody. Prior to incubation with primary antibody, the sample was separated on 15% SDS-PAGE.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SOD1 in A) HeLa mock control, B) HeLa transfected shSOD1 using SOD1 polyclonal antibody (Product # PA5-85095) using 10 µg of sample at a dilution of 1:500. Sample was then incubated with HRP-conjugated anti-rabbit IgG secondary antibody. Prior to incubation with primary antibody, the sample was separated on 15% SDS-PAGE.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of SOD1 was performed by separating 30 µg of various whole cell extracts by 15% SDS-PAGE. Proteins were transferred to a membrane and probed with a SOD1 Polyclonal Antibody (Product # PA5-85095) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using SOD1 Polyclonal Antibody (Product # PA5-85095). Sample (50 µg of whole cell lysate). Lane A: Rat brain. 15% SDS PAGE. SOD1 Polyclonal Antibody (Product # PA5-85095) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using SOD1 Polyclonal Antibody (Product # PA5-85095). Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with SOD1 Polyclonal Antibody (Product # PA5-85095) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using SOD1 Polyclonal Antibody (Product # PA5-85095). Sample (50 µg of whole cell lysate). Lane A: mouse brain. 15% SDS PAGE. SOD1 Polyclonal Antibody (Product # PA5-85095) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SOD1 Polyclonal Antibody detects SOD1 protein by western blot analysis. Whole cell extracts (30 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with SOD1 Polyclonal Antibody (Product # PA5-85095) at a dilution of 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis (Fig a) of SOD1 was performed on cell extracts (30µg of lysate) of HeLa wild type (Lane 1) and SOD1 knockout (Lane 2). The blot was probed with the Anti-SOD1 Polyclonal Antibody (Product # PA5-85095, 1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Densitometric analysis of this Western blot is shown in the histogram (Fig b). Loss of signal upon CRISPR mediated knockout (KO) confirms that antibody is specific to SOD1..
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-SOD1 Polyclonal Antibody, (Product # PA5-85095) and a ~16 kDa band corresponding to SOD1 was observed across the cell lines tested. Whole cell extracts (30 µg lysate) of HAEC (Lane1), A549 (Lane 2), HCT 116 (Lane 3), MCF-7 (Lane 4) and U-2OS (Lane 5) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blots were probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005)..
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ELISA detection of SOD1 using SOD1 Polyclonal Antibody (Product # PA5-85095) for capture at a concentration of 5 µg/mL and a SOD1 Polyclonal Antibody for detection at a concentration of 1.5 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SOD1 Polyclonal Antibody detects SOD1 protein at nucleus by immunofluorescent analysis. Sample: U-87 MG cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SOD1 protein stained by SOD1 Polyclonal Antibody (Product # PA5-85095) diluted at 1:500. Red: beta Tubulin 3/ TUJ1 protein stained by beta-3 Tubulin Polyclonal Antibody diluted at 1:200.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SOD1 Polyclonal Antibody detects SOD1 protein at cytosol on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. SOD1 Polyclonal Antibody (Product # PA5-85095) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) photomicrographs of paraffin-embedded mouse fetal brain. Green: SOD1 Polyclonal Antibody (Product # PA5-85095) diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488). Red: beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:100. The signal was developed using goat anti-mouse IgG antibody (Dylight594). Blue: Nuclear staining with Hoechst 33342. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ELISA analysis of SOD1 using SOD1 polyclonal antibody (Product # PA5-85095) at a dilution of 1.5 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 6 Protein expression of AMPK, NFkappaB-p65, IkappaB-alpha, SOD1 and CAT in skin tissues. ( A ) relative expression levels of proteins; ( B ) protein banding map. * p < 0.05 compared to the UVB group; ** p < 0.01 compared to the UVB group; *** p < 0.001 compared to the UVB group. Abbreviations : VC+UVB, mice treated with vitamin C(300mg/kg) and UVB irradiation; NMN+UVB, mice treated with nicotinamide mononucleotide (300mg/kg) and UVB irradiation.
