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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [5]
- Immunocytochemistry [1]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- MA5-15520 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SOD1 Monoclonal Antibody (6F5)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-15520 targets SOD1 in FACS, ICC, IF and WB applications and shows reactivity with Human and mouse samples.
- Antibody clone number
- 6F5
- Concentration
- Conc. Not Determined
Submitted references Nuclear Phospho-SOD1 Protects DNA from Oxidative Stress Damage in Amyotrophic Lateral Sclerosis.
Dysregulation of Mitochondrial Functions and Osteogenic Differentiation in Cisd2-Deficient Murine Induced Pluripotent Stem Cells.
Bordoni M, Pansarasa O, Dell'Orco M, Crippa V, Gagliardi S, Sproviero D, Bernuzzi S, Diamanti L, Ceroni M, Tedeschi G, Poletti A, Cereda C
Journal of clinical medicine 2019 May 22;8(5)
Journal of clinical medicine 2019 May 22;8(5)
Dysregulation of Mitochondrial Functions and Osteogenic Differentiation in Cisd2-Deficient Murine Induced Pluripotent Stem Cells.
Tsai PH, Chien Y, Chuang JH, Chou SJ, Chien CH, Lai YH, Li HY, Ko YL, Chang YL, Wang CY, Liu YY, Lee HC, Yang CH, Tsai TF, Lee YY, Chiou SH
Stem cells and development 2015 Nov 1;24(21):2561-76
Stem cells and development 2015 Nov 1;24(21):2561-76
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SOD1 using SOD1 monoclonal antibody (Product # MA5-15520) in HeLa (1), NIH/3T3 (2), A549 (3) and A431 (4) cell lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SOD1 using SOD1 monoclonal antibody (Product # MA5-15520) in HeLa (1), NIH/3T3 (2), A549 (3) and A431 (4) cell lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis (Fig a) of SOD1 was performed on cell extracts (30µg of lysate) of HeLa wild type (Lane 1) and SOD1 knockout (Lane 2). The blot was probed with the Anti-SOD1 Monoclonal Antibody (Product # MA5-15520, 1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). using the iBright FL 1000 (Product # A32752). Loss of signal upon CRISPR mediated knockout (KO) confirms that antibody is specific to SOD1..
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-SOD1 Monoclonal Antibody, (Product # MA5-15520) and a ~16 kDa band corresponding to SOD1 was observed across the cell lines tested. Whole cell extracts (30 µg lysate) of HAEC (Lane1), A549 (Lane 2), HCT 116 (Lane 3), MCF-7 (Lane 4) and U-2OS (Lane 5) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005)..
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SOD1 using SOD1 monoclonal antibody (Product # MA5-15520) in HeLa (1), NIH/3T3 (2), A549 (3) and A431 (4) cell lysate.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of 3T3-L1 cells using SOD1 monoclonal antibody (Product # MA5-15520) (Green). Blue: DRAQ5 fluorescent DNA dye.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of A431 cells using SOD1 monoclonal antibody (Product # MA5-15520) (green) and negative control (purple).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
