Antibody data
- Product number
- PA5-23245
- Provider
- Invitrogen Antibodies
- Product name
- Anti-SOD1 Polyclonal Antibody
- Provider product page
- Invitrogen Antibodies - PA5-23245
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse, Rat, Bovine
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1.0 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Supportive validation
- Submitted by
-
Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SOD in liver lysate of 1) human, 2) mouse and 3) rat using a SOD polyclonal antibody (Product # PA5-23245) at 0.025 µg/mL.
Supportive validation
- Submitted by
-
Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SOD1 was performed by loading 30 µg of HeLa (Lane 1), HeLa - SOD1 knockout (Lane 2) whole cell lysate. The blot was probed with Anti-SOD1 Polyclonal Antibody (Product # PA5-23245, 0.1 µg/mL) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). Loss of signal upon CRISPR mediated knockout (KO) confirms that antibody is specific to SOD1.
Supportive validation
- Submitted by
-
Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts of HEK-293 (Lane 1), HEK-293 treated with H2O2 (10uM, 6hrs) (Lane 2), HeLa (Lane 3), MCF-7 (Lane 4), and SH-SY5Y (Lane 5). The blot was probed with SOD1 Polyclonal Antibody (Product # PA5-23245, 0.1µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 18kDa band corresponding to SOD1 was observed across the panel and was upregulated upon H2O2 treatment in HEK-293 cell lines.
Supportive validation
- Submitted by
-
Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of SOD in paraffin-embedded formalin-fixed human liver tissue using an isotype control (top) and a SOD polyclonal antibody (Product # PA5-23245) (bottom) at 5 µg/mL.
Supportive data in Antibodypedia
