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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [4]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-23245 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SOD1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse, Rat, Bovine
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1.0 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Effects of BPA, BPS, and BPF on Oxidative Stress and Antioxidant Enzyme Expression in Bovine Oocytes and Spermatozoa.
Obesity is a risk factor for preoperative hypoxemia in Stanford A acute aortic dissection.
Remifentanil preconditioning protects against hypoxia-induced senescence and necroptosis in human cardiac myocytes in vitro.
Nguyen M, Sabry R, Davis OS, Favetta LA
Genes 2022 Jan 14;13(1)
Genes 2022 Jan 14;13(1)
Obesity is a risk factor for preoperative hypoxemia in Stanford A acute aortic dissection.
Wu Z, Wang Z, Wu H, Hu R, Ren W, Hu Z, Chang J
Medicine 2020 Mar;99(11):e19186
Medicine 2020 Mar;99(11):e19186
Remifentanil preconditioning protects against hypoxia-induced senescence and necroptosis in human cardiac myocytes in vitro.
Lewinska A, Adamczyk-Grochala J, Bloniarz D, Horeczy B, Zurek S, Kurowicki A, Woloszczuk-Gebicka B, Widenka K, Wnuk M
Aging 2020 Jun 25;12(14):13924-13938
Aging 2020 Jun 25;12(14):13924-13938
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SOD in liver lysate of 1) human, 2) mouse and 3) rat using a SOD polyclonal antibody (Product # PA5-23245) at 0.025 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SOD1 in liver lysate in the 1) human, 2) mouse, and 3) rat. Samples were incubated in SOD1 polyclonal antibody (Product # PA5-23245) using a dilution of 0.25 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SOD1 was performed by loading 30 µg of HeLa (Lane 1), HeLa - SOD1 knockout (Lane 2) whole cell lysate. The blot was probed with Anti-SOD1 Polyclonal Antibody (Product # PA5-23245, 0.1 µg/mL) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). Loss of signal upon CRISPR mediated knockout (KO) confirms that antibody is specific to SOD1.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts of HEK-293 (Lane 1), HEK-293 treated with H2O2 (10uM, 6hrs) (Lane 2), HeLa (Lane 3), MCF-7 (Lane 4), and SH-SY5Y (Lane 5). The blot was probed with SOD1 Polyclonal Antibody (Product # PA5-23245, 0.1µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 18kDa band corresponding to SOD1 was observed across the panel and was upregulated upon H2O2 treatment in HEK-293 cell lines.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of SOD1 in formalin-fixed paraffin-embedded human liver tissue. Samples were incubated in SOD1 polyclonal antibody (Product # PA5-23245) using a dilution of 5 µg/mL. Isotype control (top) and this antibody (bottom).
