Antibody data
- Antibody Data
- Antigen structure
- References [9]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- PA1345 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-Superoxide Dismutase 1/SOD1 Antibody
- Antibody type
- Polyclonal
- Description
- Polyclonal antibody for CU ZN SOD/SOD1 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. CU ZN SOD/SOD1 information: Molecular Weight: 15936 MW; Subcellular Localization: Cytoplasm. Nucleus. Predominantly cytoplasmic; the pathogenic variants ALS1 Arg-86 and Ala-94 gradually aggregates and accumulates in mitochondria.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references Fucoxanthin ameliorated myocardial fibrosis in STZ-induced diabetic rats and cell hypertrophy in HG-induced H9c2 cells by alleviating oxidative stress and restoring mitophagy.
Prenatal hypothyroidism diminished exogenous NO-mediated diastolic effects in fetal rat thoracic aorta smooth muscle via increased oxidative stress.
Acacetin alleviates myocardial ischaemia/reperfusion injury by inhibiting oxidative stress and apoptosis via the Nrf-2/HO-1 pathway.
Fucoxanthin regulates Nrf2 signaling to decrease oxidative stress and improves renal fibrosis depending on Sirt1 in HG-induced GMCs and STZ-induced diabetic rats.
Doxorubicin cardiomyopathy is ameliorated by acacetin via Sirt1-mediated activation of AMPK/Nrf2 signal molecules.
The Natural Flavone Acacetin Confers Cardiomyocyte Protection Against Hypoxia/Reoxygenation Injury via AMPK-Mediated Activation of Nrf2 Signaling Pathway.
Discovery of a head-to-tail cyclic peptide as the Keap1-Nrf2 protein-protein interaction inhibitor with high cell potency.
Effect of exenatide on the cardiac expression of adiponectin receptor 1 and NADPH oxidase subunits and heart function in streptozotocin-induced diabetic rats.
Combination therapy with losartan and pioglitazone additively reduces renal oxidative and nitrative stress induced by chronic high fat, sucrose, and sodium intake.
Zheng D, Chen L, Li G, Jin L, Wei Q, Liu Z, Yang G, Li Y, Xie X
Food & function 2022 Sep 22;13(18):9559-9575
Food & function 2022 Sep 22;13(18):9559-9575
Prenatal hypothyroidism diminished exogenous NO-mediated diastolic effects in fetal rat thoracic aorta smooth muscle via increased oxidative stress.
Guo J, Yu X, Liu Y, Lu L, Zhu D, Zhang Y, Li L, Zhang P, Gao Q, Lu X, Sun M
Reproductive toxicology (Elmsford, N.Y.) 2022 Oct;113:52-61
Reproductive toxicology (Elmsford, N.Y.) 2022 Oct;113:52-61
Acacetin alleviates myocardial ischaemia/reperfusion injury by inhibiting oxidative stress and apoptosis via the Nrf-2/HO-1 pathway.
Wu C, Chen RL, Wang Y, Wu WY, Li G
Pharmaceutical biology 2022 Dec;60(1):553-561
Pharmaceutical biology 2022 Dec;60(1):553-561
Fucoxanthin regulates Nrf2 signaling to decrease oxidative stress and improves renal fibrosis depending on Sirt1 in HG-induced GMCs and STZ-induced diabetic rats.
Yang G, Li Q, Peng J, Jin L, Zhu X, Zheng D, Zhang Y, Wang R, Song Y, Hu W, Xie X
European journal of pharmacology 2021 Dec 15;913:174629
European journal of pharmacology 2021 Dec 15;913:174629
Doxorubicin cardiomyopathy is ameliorated by acacetin via Sirt1-mediated activation of AMPK/Nrf2 signal molecules.
Wu WY, Cui YK, Hong YX, Li YD, Wu Y, Li G, Li GR, Wang Y
Journal of cellular and molecular medicine 2020 Oct;24(20):12141-12153
Journal of cellular and molecular medicine 2020 Oct;24(20):12141-12153
The Natural Flavone Acacetin Confers Cardiomyocyte Protection Against Hypoxia/Reoxygenation Injury via AMPK-Mediated Activation of Nrf2 Signaling Pathway.
Wu WY, Li YD, Cui YK, Wu C, Hong YX, Li G, Wu Y, Jie LJ, Wang Y, Li GR
Frontiers in pharmacology 2018;9:497
Frontiers in pharmacology 2018;9:497
Discovery of a head-to-tail cyclic peptide as the Keap1-Nrf2 protein-protein interaction inhibitor with high cell potency.
Lu MC, Jiao Q, Liu T, Tan SJ, Zhou HS, You QD, Jiang ZY
European journal of medicinal chemistry 2018 Jan 1;143:1578-1589
European journal of medicinal chemistry 2018 Jan 1;143:1578-1589
Effect of exenatide on the cardiac expression of adiponectin receptor 1 and NADPH oxidase subunits and heart function in streptozotocin-induced diabetic rats.
Guo Z, Qi W, Yu Y, Du S, Wu J, Liu J
Diabetology & metabolic syndrome 2014 Feb 28;6(1):29
Diabetology & metabolic syndrome 2014 Feb 28;6(1):29
Combination therapy with losartan and pioglitazone additively reduces renal oxidative and nitrative stress induced by chronic high fat, sucrose, and sodium intake.
Kong X, Zhang Y, Wu HB, Li FX, Zhang DY, Su Q
Oxidative medicine and cellular longevity 2012;2012:856085
Oxidative medicine and cellular longevity 2012;2012:856085
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- Western blot analysis of SOD1 using anti-SOD1 antibody (PA1345). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: mouse liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOD1 antigen affinity purified polyclonal antibody (Catalog # PA1345) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SOD1 at approximately 18KD. The expected band size for SOD1 is at 18KD.
- Additional image