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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
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- Product number
- AF3418 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse SOD1/Cu-Zn SOD Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human and mouse SOD1/Cu-Zn SOD in Western blots. Detects rat SOD1, but Catalog # AF3787 is recommended.
- Reactivity
- Human, Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P00441- Isotype
- IgG
- Vial size
- 50 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Neuroprotective and neuritogenic activities of novel multimodal iron-chelating drugs in motor-neuron-like NSC-34 cells and transgenic mouse model of amyotrophic lateral sclerosis.
Kupershmidt L, Weinreb O, Amit T, Mandel S, Carri MT, Youdim MB
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2009 Nov;23(11):3766-79
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2009 Nov;23(11):3766-79
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human/Mouse SOD1/Cu-Zn SOD by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.2 µg/mL Goat Anti-Human/Mouse SOD1/Cu-Zn SOD Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3418) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, recombinant human SOD1, SOD2, and SOD3 (1 ng/lane) were included. A specific band for SOD1/Cu-Zn SOD was detected at approximately 16-19 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human SOD1/Cu-Zn SOD by Simple WesternTM. Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for SOD1/Cu-Zn SOD at approximately 25 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse SOD1/Cu-Zn SOD Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3418) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human SOD1/Cu-Zn SOD by Simple WesternTM. Simple Western lane view shows lysates of A549 human lung carcinoma cell line and SK-BR-3 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for SOD1/Cu-Zn SOD at approximately 24 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse SOD1/Cu-Zn SOD Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3418) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
