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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- MAB3418 - Provider product page
- Provider
- R&D Systems
- Product name
- Human SOD1/Cu-Zn SOD Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human SOD1/Cu-Zn SOD in Western blots. In Western blots, no cross-reactivity with recombinant human (rh) SOD2 or rhSOD3 is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P00441- Isotype
- IgG
- Antibody clone number
- 348808
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Activation of EGFR by small compounds through coupling the generation of hydrogen peroxide to stable dimerization of Cu/Zn SOD1.
Sakanyan V, Hulin P, Alves de Sousa R, Silva VA, Hambardzumyan A, Nedellec S, Tomasoni C, Logé C, Pineau C, Roussakis C, Fleury F, Artaud I
Scientific reports 2016 Feb 17;6:21088
Scientific reports 2016 Feb 17;6:21088
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human SOD1/ Cu-Zn SOD by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and Ramos human Burkitt's lymphoma cell line. PVDF membrane was probed with 0.5 µg/mL Mouse Anti-Human SOD1/Cu-Zn SOD Monoclonal Antibody (Catalog # MAB3418) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human SOD1, SOD2, and SOD3 (1 ng) were included. A specific band for SOD1/Cu-Zn SOD was detected at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- SOD1/Cu-Zn SOD in HeLa Human Cell Line. SOD1/Cu-Zn SOD was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human SOD1/Cu-Zn SOD Monoclonal Antibody (Catalog # MAB3418) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
