Antibody data
- Antibody Data
- Antigen structure
- References [11]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AF1819 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal Klotho Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse Klotho in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant mouse beta-Klotho is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Wnt/β-catenin/RAS signaling mediates age-related renal fibrosis and is associated with mitochondrial dysfunction.
Release of insulin produced by the choroid plexis is regulated by serotonergic signaling.
Secreted Klotho Attenuates Inflammation-Associated Aortic Valve Fibrosis in Senescence-Accelerated Mice P1.
Klotho expression is a prerequisite for proper muscle stem cell function and regeneration of skeletal muscle.
Persistent fibroblast growth factor 23 signalling in the parathyroid glands for secondary hyperparathyroidism in mice with chronic kidney disease.
Antiaging Gene Klotho Attenuates Pancreatic β-Cell Apoptosis in Type 1 Diabetes.
FGF23 suppresses chondrocyte proliferation in the presence of soluble α-Klotho both in vitro and in vivo.
Expression of klotho mRNA and protein in rat brain parenchyma from early postnatal development into adulthood.
Loss of Klotho contributes to kidney injury by derepression of Wnt/β-catenin signaling.
Vitamin D receptor agonists increase klotho and osteopontin while decreasing aortic calcification in mice with chronic kidney disease fed a high phosphate diet.
Circulating αKlotho influences phosphate handling by controlling FGF23 production.
Miao J, Liu J, Niu J, Zhang Y, Shen W, Luo C, Liu Y, Li C, Li H, Yang P, Liu Y, Hou FF, Zhou L
Aging cell 2019 Oct;18(5):e13004
Aging cell 2019 Oct;18(5):e13004
Release of insulin produced by the choroid plexis is regulated by serotonergic signaling.
Mazucanti CH, Liu QR, Lang D, Huang N, O'Connell JF, Camandola S, Egan JM
JCI insight 2019 Dec 5;4(23)
JCI insight 2019 Dec 5;4(23)
Secreted Klotho Attenuates Inflammation-Associated Aortic Valve Fibrosis in Senescence-Accelerated Mice P1.
Chen J, Fan J, Wang S, Sun Z
Hypertension (Dallas, Tex. : 1979) 2018 May;71(5):877-885
Hypertension (Dallas, Tex. : 1979) 2018 May;71(5):877-885
Klotho expression is a prerequisite for proper muscle stem cell function and regeneration of skeletal muscle.
Ahrens HE, Huettemeister J, Schmidt M, Kaether C, von Maltzahn J
Skeletal muscle 2018 Jul 4;8(1):20
Skeletal muscle 2018 Jul 4;8(1):20
Persistent fibroblast growth factor 23 signalling in the parathyroid glands for secondary hyperparathyroidism in mice with chronic kidney disease.
Kawakami K, Takeshita A, Furushima K, Miyajima M, Hatamura I, Kuro-O M, Furuta Y, Sakaguchi K
Scientific reports 2017 Jan 17;7:40534
Scientific reports 2017 Jan 17;7:40534
Antiaging Gene Klotho Attenuates Pancreatic β-Cell Apoptosis in Type 1 Diabetes.
Lin Y, Sun Z
Diabetes 2015 Dec;64(12):4298-311
Diabetes 2015 Dec;64(12):4298-311
FGF23 suppresses chondrocyte proliferation in the presence of soluble α-Klotho both in vitro and in vivo.
Kawai M, Kinoshita S, Kimoto A, Hasegawa Y, Miyagawa K, Yamazaki M, Ohata Y, Ozono K, Michigami T
The Journal of biological chemistry 2013 Jan 25;288(4):2414-27
The Journal of biological chemistry 2013 Jan 25;288(4):2414-27
Expression of klotho mRNA and protein in rat brain parenchyma from early postnatal development into adulthood.
Clinton SM, Glover ME, Maltare A, Laszczyk AM, Mehi SJ, Simmons RK, King GD
Brain research 2013 Aug 21;1527:1-14
Brain research 2013 Aug 21;1527:1-14
Loss of Klotho contributes to kidney injury by derepression of Wnt/β-catenin signaling.
Zhou L, Li Y, Zhou D, Tan RJ, Liu Y
Journal of the American Society of Nephrology : JASN 2013 Apr;24(5):771-85
Journal of the American Society of Nephrology : JASN 2013 Apr;24(5):771-85
Vitamin D receptor agonists increase klotho and osteopontin while decreasing aortic calcification in mice with chronic kidney disease fed a high phosphate diet.
Lau WL, Leaf EM, Hu MC, Takeno MM, Kuro-o M, Moe OW, Giachelli CM
Kidney international 2012 Dec;82(12):1261-70
Kidney international 2012 Dec;82(12):1261-70
Circulating αKlotho influences phosphate handling by controlling FGF23 production.
Smith RC, O'Bryan LM, Farrow EG, Summers LJ, Clinkenbeard EL, Roberts JL, Cass TA, Saha J, Broderick C, Ma YL, Zeng QQ, Kharitonenkov A, Wilson JM, Guo Q, Sun H, Allen MR, Burr DB, Breyer MD, White KE
The Journal of clinical investigation 2012 Dec;122(12):4710-5
The Journal of clinical investigation 2012 Dec;122(12):4710-5
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Mouse Klotho by Western Blot. Detection of Mouse Klotho by Western Blot.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Mouse Klotho ELISA Standard Curve. Mouse Klotho ELISA Standard Curve.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Klotho in Mouse Kidney. Klotho in Mouse Kidney.