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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- Immunohistochemistry [5]
- Other assay [1]
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- Product number
- PA5-88303 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Klotho Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: MPASAPPRRP RPPPPSLSLL LVLLGLGGRR LRAEPGDGAQ TWARFSRPPA PEAAGLFQGT FPDGFLWAVG SAAYQTEGGW QQHGKGASIW DTFTHHPLAP PGDSRNASLP LGAPSPLQPA TGDVASDSYN NVFRDTEALR ELGVTHYRFS ISWARVLPNG SAGVPNREGL RYYRRLLERL RELGVQPVVT LYHWDLPQRL; Positive Samples: DU145, Mouse kidney, Mouse brain, Rat kidney; Cellular Location: Cell membrane, Single-pass type I membrane protein
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 2.32 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Effects of chronic kidney disease on cognitive function and α-klotho expression in hippocampus.
Huang M, Li G, Tan J, Huang M, Huang F, Ma R, Xiao Y, Wang J
Translational andrology and urology 2022 Aug;11(8):1157-1168
Translational andrology and urology 2022 Aug;11(8):1157-1168
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts of various cell lines, using KL Polyclonal antibody (Product # PA5-88303) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 5s.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Klotho in extracts of various cell lines using Klotho Polyclonal Antibody (Product # PA5-88303) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-Klotho Polyclonal Antibody (Product # PA5-88303) and a 116 kDa band corresponding to Klotho was observed across cell lines. Whole cell extracts (60 µg lysate) of SW480 (Lane 1), SW480 treated with protein translocator inhibitor(PTI) for 4 hrs (Lane 2), PANC-1 (Lane 3), PANC-1 treated with protein translocator inhibitor(PTI) for 4 hrs (Lane 4) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:20000 using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076). The relative expression of Klotho could be seen in the positive cell model SW480 over the low expressing model PANC-1 as reported at a band of 116 kDa molecular weight specific to Klotho.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Klotho in paraffin-embedded human lung cancer using Klotho Polyclonal Antibody (Product # PA5-88303) at a dilution of 1:200.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Klotho in paraffin-embedded human placenta using Klotho Polyclonal Antibody (Product # PA5-88303) at a dilution of 1:200.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry-Immunofluorescence analysis of Klotho was performed in rat kidney tissue using Klotho Polyclonal Antibody (Product # PA5-88303) at a dilution of 1:100. Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry-Immunofluorescence analysis of Klotho was performed in human placenta tissue using Klotho Polyclonal Antibody (Product # PA5-88303) at a dilution of 1:100. Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry-Immunofluorescence analysis of Klotho was performed in mouse kidney tissue using Klotho Polyclonal Antibody (Product # PA5-88303) at a dilution of 1:100. Blue: DAPI for nuclear staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- The expression of hippocampal alpha-KL was reduced in CKD rats. (A, B) The Western blotting assay revealed decreased alpha-KL expression in the hippocampus of the CKD rats. (C) Similarly, RT-qPCR showed that the relative expression of KL mRNA was lower in the CKD rat hippocampal tissues; internal reference: beta-actin, n=3. (D-F) The results of IHC staining against alpha-KL. (D) Representative IHC images. The expression of alpha-KL was significantly decreased in the CA3 region of the hippocampus of the CKD rats, which was quantified as positive cell count (E) and mean optical density (F); n=5, 7. *P
