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antibody from Invitrogen Antibodies
Targeting: H3C2
H3/l, H3FL, HIST1H3B
Western blot
ELISA
Immunocytochemistry Immunohistochemistry Chromatin Immunoprecipitation Other assayAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [2]
- Immunohistochemistry [3]
- Chromatin Immunoprecipitation [1]
- Other assay [2]
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- Product number
- PA5-116985 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- H3R8me2a Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Positive Samples: HeLa, NIH/3T3 Immunogen sequence: TARKS
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 μL
- Concentration
- 0.37 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence analysis of H3R8me2a in 293T cells using H3R8me2a Polyclonal Antibody (Product # PA5-116985) at a dilution of 1:100. Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of H3R8me2a in 293T cells. Samples were incubated with H3R8me2a Polyclonal antibody (Product # PA5-116985). Blue: DAPI for nuclear staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of H3R8me2a in paraffin-embedded human colon. Samples were incubated with H3R8me2a Polyclonal antibody (Product # PA5-116985) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of H3R8me2a in paraffin-embedded mouse testis. Samples were incubated with H3R8me2a Polyclonal antibody (Product # PA5-116985) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of H3R8me2a in paraffin-embedded rat testis. Samples were incubated with H3R8me2a Polyclonal antibody (Product # PA5-116985) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ChIP analysis of H3R8me2a in extracts of HeLa cells. Samples were incubated with H3R8me2a Polyclonal antibody (Product # PA5-116985) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Dot-blot analysis of all sorts of methylation peptides using H3R8me2a Polyclonal Antibody (Product # PA5-116985).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Dot blot analysis of all sorts of methylation peptides using Asymmetric DiMethyl-Histone H3-R8 polyclonal antibody (Product # PA5-116985).
