AF2430

antibody from Novus Biologicals

Targeting: PRSS50 CT20, TSP50

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Antibody Data

Product number

AF2430

Provider

Novus Biologicals

Product name

Goat Polyclonal TSP50 Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects human TSP50 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant mouse TSP50 is observed.

Reactivity

Human

Host

Goat

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human TSP50 by Western Blot. Western blot shows lysates of human testis tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human TSP50 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2430) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for TSP50 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.