17190002-0.1mg

antibody from Novus Biologicals

Targeting: PRSS50 CT20, TSP50

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

17190002-0.1mg

Provider

Novus Biologicals

Product name

Rabbit Polyclonal TSP50 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. This product is specific for Human TSP50.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

0.1 mg

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: TSP50 Antibody [17190002] - Samples: Lane 1, Marker [kDa]: 219, 111, 83, 48, 32, 26, 17 Lane 2, RT-4 Lane 3, U-251MG sp Lane 4, Human Plasma Lane 5, Liver Lane 6, Tonsil, Target weight [kDa]: 43 Validation score: 2 Validation description: Supportive - Band of predicted size in kDa (+/-20%) with additional bands present.

ELISA

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

ELISA: TSP50 Antibody [17190002]

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: TSP50 Antibody [17190002] - Strong cytoplasmic positivity was seen in primary spermatocytes of the seminiferous tubules of the testes, neurons of the cerebral cortex and many hematopoeitic cells of the bone marrow. Most mucosal surfaces and glands showed moderate or some times weak positivity. Biliary ductal cells and gall bladder mucosal cells showed strong accentuation of the luminal cell membrane. Most other normal tissues showed moderate or weak positivity. More consistent and stronger expression was seen in malignancies. Strong or moderate positivity was seen in prostatic, testicular and colo-rectal cancers. Many pancreatic, prostatic and some testicular cancers showed strong cytoplasmic granularity. Strong or moderate positivity was also seen in malignant carcinoid, renal and urothelial cancers. Most other cancers showed moderate or sometimes weak positivity. Image and statement courtesy of the Human Protein Atlas (HPA).