Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- ELISA [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- H00002624-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00002624-M01, RRID:AB_539064
- Product name
- Mouse Monoclonal GATA-2 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. GATA2 - GATA binding protein 2
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Single-cell epigenomic variability reveals functional cancer heterogeneity.
Litzenburger UM, Buenrostro JD, Wu B, Shen Y, Sheffield NC, Kathiria A, Greenleaf WJ, Chang HY
Genome biology 2017 Jan 24;18(1):15
Genome biology 2017 Jan 24;18(1):15
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GATA-2 Antibody (2D11) [H00002624-M01] - Analysis of GATA2 over-expressed 293 cell line, cotransfected with GATA2 Validated Chimera RNAi ( Cat # H00002624-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with GATA2 monoclonal antibody (M01), clone 2D11 (Cat # H00002624-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GATA-2 Antibody (2D11) [H00002624-M01] - Analysis of GATA2 expression in transfected 293T cell line by GATA2 monoclonal antibody (M01), clone 2D11.Lane 1: GATA2 transfected lysate(50.5 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GATA-2 Antibody (2D11) [H00002624-M01] - GATA2 monoclonal antibody (M01), clone 2D11 Analysis of GATA2 expression in Hela S3 NE.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: GATA-2 Antibody (2D11) [H00002624-M01] - Detection limit for recombinant GST tagged GATA2 is approximately 0.03ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: GATA-2 Antibody (2D11) [H00002624-M01] - Analysis of monoclonal antibody to GATA2 on HeLa cell. Antibody concentration 10 ug/ml.